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Items: 8

1.

Neuronal HSF-1 activity coordinates fat desaturation across tissues in C. elegans via TGF-b/BMP signalling

(Submitter supplied) To survive elevated temperatures, ectotherms adjust the fluidity of membranes by fine-tuning lipid desaturation levels in a process that has been previously described to be cell-autonomous. We have discovered that, in Caenorhabditis elegans, the neuronal over-expression of the master regulator of the conserved heat shock response (HSR), Hsf-1, causes extensive fat remodelling in peripheral tissues. These changes include a decrease in fat desaturase expression in the gut, and a shift in the saturation levels of fatty acids in the plasma membrane, in line with ectothermic adaptive responses. We have identified the cGMP receptor TAX-2/TAX-4 and TGF-β/BMP signalling, as key players in signalling across tissues. We also find that gradual increments in ambient temperature result in HSR activation exclusively in wild-type head neurons. This is the first study to suggest that a thermostat-based mechanism can non-cell autonomously coordinate membrane fluidity in response to warm temperatures across tissues in multicellular animals.
Organism:
Caenorhabditis elegans
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18245
6 Samples
Download data: XLSX
Series
Accession:
GSE145123
ID:
200145123
2.

Illumina HiSeq 2500 (Caenorhabditis elegans)

Organism:
Caenorhabditis elegans
475 Series
8107 Samples
Download data
Platform
Accession:
GPL18245
ID:
100018245
3.

AGD1289 replicate 3

Organism:
Caenorhabditis elegans
Source name:
1000 young adult worms
Platform:
GPL18245
Series:
GSE145123
Download data
Sample
Accession:
GSM4306940
ID:
304306940
4.

AGD1289 replicate 2

Organism:
Caenorhabditis elegans
Source name:
1000 young adult worms
Platform:
GPL18245
Series:
GSE145123
Download data
Sample
Accession:
GSM4306939
ID:
304306939
5.

AGD1289 replicate 1

Organism:
Caenorhabditis elegans
Source name:
1000 young adult worms
Platform:
GPL18245
Series:
GSE145123
Download data
Sample
Accession:
GSM4306938
ID:
304306938
6.

N2 replicate 3

Organism:
Caenorhabditis elegans
Source name:
1000 young adult worms
Platform:
GPL18245
Series:
GSE145123
Download data
Sample
Accession:
GSM4306937
ID:
304306937
7.

N2 replicate 2

Organism:
Caenorhabditis elegans
Source name:
1000 young adult worms
Platform:
GPL18245
Series:
GSE145123
Download data
Sample
Accession:
GSM4306936
ID:
304306936
8.

N2 replicate 1

Organism:
Caenorhabditis elegans
Source name:
1000 young adult worms
Platform:
GPL18245
Series:
GSE145123
Download data
Sample
Accession:
GSM4306935
ID:
304306935
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Supplemental Content

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