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Items: 10

1.

Embedding siRNA sequences targeting Apolipoprotein B100 in shRNA and miRNA scaffolds results in differential processing and in vivo efficacy

(Submitter supplied) Purpose: Identical predicted small interfering RNA (siRNA) sequences targeting Apolipoprotein B100 (siApoB) were embedded in shRNA (shApoB) or miRNA (miApoB) scaffolds and a direct compariso of the possible aspecific off-target effects in vivo was performed. Next generation sequencing (NGS) of small RNAs originating from shApoB- or miApoB-transfected cells revealed substantial differences in processing, resulting in different siApoB length, 5’ and 3’ cleavage sites and abundance of the guide or passenger strands. more...
Organism:
Mus musculus; Homo sapiens
Type:
Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing
Platforms:
GPL11154 GPL13112
7 Samples
Download data: PDF, TXT
Series
Accession:
GSE39187
ID:
200039187

PubMed Full text in PMC Similar studies SRA Run Selector

2.

Illumina HiSeq 2000 (Mus musculus)

Organism:
Mus musculus
8229 Series
187279 Samples
Download data
Platform
Accession:
GPL13112
ID:
100013112

3.

Illumina HiSeq 2000 (Homo sapiens)

Organism:
Homo sapiens
10400 Series
180082 Samples
Download data
Platform
Accession:
GPL11154
ID:
100011154

4.

miApoB (small RNA)

Organism:
Homo sapiens
Source name:
miApoB
Platform:
GPL11154
Series:
GSE39187
Download data: TXT
Sample
Accession:
GSM957706
ID:
300957706

SRA Run Selector

5.

shApoB (small RNA)

Organism:
Homo sapiens
Source name:
shApoB
Platform:
GPL11154
Series:
GSE39187
Download data: TXT
Sample
Accession:
GSM957705
ID:
300957705

SRA Run Selector

6.

PBS

Organism:
Mus musculus
Source name:
PBS
Platform:
GPL13112
Series:
GSE39187
Download data
Sample
Accession:
GSM957704
ID:
300957704

SRA Run Selector

7.

miApoB

Organism:
Mus musculus
Source name:
miApoB
Platform:
GPL13112
Series:
GSE39187
Download data
Sample
Accession:
GSM957703
ID:
300957703

SRA Run Selector

8.

miScr

Organism:
Mus musculus
Source name:
miScr
Platform:
GPL13112
Series:
GSE39187
Download data
Sample
Accession:
GSM957702
ID:
300957702

SRA Run Selector

9.

shApoB

Organism:
Mus musculus
Source name:
shApoB
Platform:
GPL13112
Series:
GSE39187
Download data
Sample
Accession:
GSM957701
ID:
300957701

SRA Run Selector

10.

shScr

Organism:
Mus musculus
Source name:
shScr
Platform:
GPL13112
Series:
GSE39187
Download data
Sample
Accession:
GSM957700
ID:
300957700

SRA Run Selector

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