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Items: 1 to 20 of 21

1.

Comprehensive comparative analysis of RNA sequencing methods for degraded or low input samples

(Submitter supplied) RNA-Seq is an effective method to study the transcriptome, but can be difficult to apply to scarce or degraded RNA from fixed clinical samples, rare cell populations, or cadavers. Recent studies have proposed several methods for RNA-Seq of low quality and/or low quantity samples, but their relative merits have not been systematically analyzed. Here, we compare five such methods using a comprehensive set of metrics, relevant to applications such as transcriptome annotation, transcript discovery, and gene expression. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL11154 GPL16791
18 Samples
Download data: TXT
2.

Illumina HiSeq 2500 (Homo sapiens)

Platform
Accession:
GPL16791
ID:
100016791
3.

Illumina HiSeq 2000 (Homo sapiens)

Platform
Accession:
GPL11154
ID:
100011154
4.

Pancreas_Total

Organism:
Homo sapiens
Source name:
human normal pancreas tissue (Zyagen)
Platform:
GPL16791
Series:
GSE40705
Download data
Sample
Accession:
GSM1129245
ID:
301129245
5.

SMART

Organism:
Homo sapiens
Source name:
human CML cell line K-562 grown in tissue culture (Ambion)
Platform:
GPL11154
Series:
GSE40705
Download data
Sample
Accession:
GSM1129239
ID:
301129239
6.

Pancreas_RiboZero

Organism:
Homo sapiens
Source name:
human normal pancreas tissue (Zyagen)
Platform:
GPL16791
Series:
GSE40705
Download data
Sample
Accession:
GSM1129244
ID:
301129244
7.

Pancreas_RNaseH

Organism:
Homo sapiens
Source name:
human normal pancreas tissue (Zyagen)
Platform:
GPL16791
Series:
GSE40705
Download data
Sample
Accession:
GSM1129243
ID:
301129243
8.

FFPE_Total

Organism:
Homo sapiens
Source name:
human normal kidney section (Cybrdi)
Platform:
GPL16791
Series:
GSE40705
Download data
Sample
Accession:
GSM1129242
ID:
301129242
9.

FFPE_RiboZero

Organism:
Homo sapiens
Source name:
human normal kidney section (Cybrdi)
Platform:
GPL16791
Series:
GSE40705
Download data
Sample
Accession:
GSM1129241
ID:
301129241
10.

FFPE_RNaseH

Organism:
Homo sapiens
Source name:
human normal kidney section (Cybrdi)
Platform:
GPL16791
Series:
GSE40705
Download data
Sample
Accession:
GSM1129240
ID:
301129240
11.

NuGEN 1f

Organism:
Homo sapiens
Source name:
human CML cell line K-562 grown in tissue culture (Ambion)
Platform:
GPL11154
Series:
GSE40705
Download data: TXT
Sample
Accession:
GSM999537
ID:
300999537
12.

NuGEN 1i

Organism:
Homo sapiens
Source name:
human CML cell line K-562 grown in tissue culture (Ambion)
Platform:
GPL11154
Series:
GSE40705
Download data: TXT
Sample
Accession:
GSM999536
ID:
300999536
13.

TruSeq

Organism:
Homo sapiens
Source name:
human CML cell line K-562 grown in tissue culture (Ambion)
Platform:
GPL11154
Series:
GSE40705
Download data: TXT
Sample
Accession:
GSM999535
ID:
300999535
14.

Total

Organism:
Homo sapiens
Source name:
human CML cell line K-562 grown in tissue culture (Ambion)
Platform:
GPL11154
Series:
GSE40705
Download data: TXT
Sample
Accession:
GSM999534
ID:
300999534
15.

PolyA

Organism:
Homo sapiens
Source name:
human CML cell line K-562 grown in tissue culture (Ambion)
Platform:
GPL11154
Series:
GSE40705
Download data: TXT
Sample
Accession:
GSM999533
ID:
300999533
16.

NuGEN 100f

Organism:
Homo sapiens
Source name:
human CML cell line K-562 grown in tissue culture (Ambion)
Platform:
GPL11154
Series:
GSE40705
Download data: TXT
Sample
Accession:
GSM999532
ID:
300999532
17.

Ribo-Zero

Organism:
Homo sapiens
Source name:
human CML cell line K-562 grown in tissue culture (Ambion)
Platform:
GPL11154
Series:
GSE40705
Download data: TXT
Sample
Accession:
GSM999531
ID:
300999531
18.

RNase H NS

Organism:
Homo sapiens
Source name:
human CML cell line K-562 grown in tissue culture (Ambion)
Platform:
GPL11154
Series:
GSE40705
Download data: TXT
Sample
Accession:
GSM999530
ID:
300999530
19.

RNase H

Organism:
Homo sapiens
Source name:
human CML cell line K-562 grown in tissue culture (Ambion)
Platform:
GPL11154
Series:
GSE40705
Download data: TXT
Sample
Accession:
GSM999529
ID:
300999529
20.

DSN-lite PCR

Organism:
Homo sapiens
Source name:
human CML cell line K-562 grown in tissue culture (Ambion)
Platform:
GPL11154
Series:
GSE40705
Download data: TXT
Sample
Accession:
GSM999528
ID:
300999528
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Supplemental Content

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