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Items: 17

1.

High-efficiency RNA cloning enables quantitative measurement of miRNA expression by deep sequencing

(Submitter supplied) MiRNA-mediated regulation depends on the stoichiometry between miRNAs and their mRNA targets. To decipher dynamic function of this complex layer, it is critical to characterize individual miRNA species within a specific cellular context. Small RNA cloning followed by deep sequencing is uniquely positioned as a genome-wide profiling method to quantify miRNA expression with potentially unlimited dynamic range and provide single-nucleotide resolution for precise miRNA classification and de novo discovery. more...
Organism:
Homo sapiens; Mus musculus
Type:
Non-coding RNA profiling by high throughput sequencing
Platforms:
GPL9185 GPL13112 GPL11154
13 Samples
Download data: XLS
Series
Accession:
GSE47858
ID:
200047858

PubMed Full text in PMC Similar studies SRA Run Selector

2.

Illumina HiSeq 2000 (Mus musculus)

Organism:
Mus musculus
8229 Series
187279 Samples
Download data
Platform
Accession:
GPL13112
ID:
100013112

3.

Illumina HiSeq 2000 (Homo sapiens)

Organism:
Homo sapiens
10400 Series
180082 Samples
Download data
Platform
Accession:
GPL11154
ID:
100011154

4.

Illumina Genome Analyzer (Mus musculus)

Organism:
Mus musculus
552 Series
3728 Samples
Download data
Platform
Accession:
GPL9185
ID:
100009185

5.

optimized ligation for hela cell miRNAs

Organism:
Homo sapiens
Source name:
hela cells
Platform:
GPL11154
Series:
GSE47858
Download data
Sample
Accession:
GSM1160912
ID:
301160912

SRA Run Selector

6.

mouse hair follicle matrix miRs cloned with the previous method

Organism:
Mus musculus
Source name:
mouse hair follicles
Platform:
GPL9185
Series:
GSE47858
Download data
Sample
Accession:
GSM1160900
ID:
301160900

SRA Run Selector

7.

optimized ligation for mouse heart miRNAs

Organism:
Mus musculus
Source name:
mouse heart
Platform:
GPL13112
Series:
GSE47858
Download data
Sample
Accession:
GSM1160911
ID:
301160911

SRA Run Selector

8.

optimized ligation for mouse brain miRNAs

Organism:
Mus musculus
Source name:
mouse brain
Platform:
GPL13112
Series:
GSE47858
Download data
Sample
Accession:
GSM1160910
ID:
301160910

SRA Run Selector

9.

optimized ligation for synthetic miRs with worm total RNA, rep2

Organism:
Mus musculus
Source name:
chemically synthesized miRNAs
Platform:
GPL13112
Series:
GSE47858
Download data
Sample
Accession:
GSM1160909
ID:
301160909

SRA Run Selector

10.

optimized ligation for synthetic miRs, rep2

Organism:
Mus musculus
Source name:
chemically synthesized miRNAs
Platform:
GPL13112
Series:
GSE47858
Download data
Sample
Accession:
GSM1160908
ID:
301160908

SRA Run Selector

11.

optimized ligation for synthetic miRs with let-7 gradient, rep2

Organism:
Mus musculus
Source name:
chemically synthesized miRNAs
Platform:
GPL13112
Series:
GSE47858
Download data
Sample
Accession:
GSM1160907
ID:
301160907

SRA Run Selector

12.

optimized ligation for synthetic miRs with worm total RNA, rep1

Organism:
Mus musculus
Source name:
chemically synthesized miRNAs
Platform:
GPL13112
Series:
GSE47858
Download data
Sample
Accession:
GSM1160906
ID:
301160906

SRA Run Selector

13.

optimized ligation for synthetic miRs, rep 1

Organism:
Mus musculus
Source name:
chemically synthesized miRNAs
Platform:
GPL13112
Series:
GSE47858
Download data
Sample
Accession:
GSM1160905
ID:
301160905

SRA Run Selector

14.

optimized ligation for synthetic miRs with let-7 gradient, rep1

Organism:
Mus musculus
Source name:
chemically synthesized miRNAs
Platform:
GPL13112
Series:
GSE47858
Download data
Sample
Accession:
GSM1160904
ID:
301160904

SRA Run Selector

15.

use of splint ligation in cloning synthetic miRs

Organism:
Mus musculus
Source name:
chemically synthesized miRNAs
Platform:
GPL13112
Series:
GSE47858
Download data
Sample
Accession:
GSM1160903
ID:
301160903

SRA Run Selector

16.

use of Thermostable ligase in cloning synthetic miRs

Organism:
Mus musculus
Source name:
chemically synthesized miRNAs
Platform:
GPL13112
Series:
GSE47858
Download data
Sample
Accession:
GSM1160902
ID:
301160902

SRA Run Selector

17.

synthetic miRs cloned with the previous method

Organism:
Mus musculus
Source name:
chemically synthesized miRNAs
Platform:
GPL13112
Series:
GSE47858
Download data
Sample
Accession:
GSM1160901
ID:
301160901

SRA Run Selector

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