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Items: 19

1.

Impact of library preparation on downstream analysis and interpretation of RNA-seq data: comparison between Illumina PolyA and NuGEN Ovation protocol

(Submitter supplied) Objectives: The sequencing by the PolyA selection is the most common approach for library preparation. With limited amount or degraded RNA, alternative protocols such as the NuGEN have been developed. However, it is not yet clear how the different library preparations affect the downstream analyses of the broad applications of RNA sequencing. Methods and Materials: Eight human mammary epithelial cell (HMEC) lines with high quality RNA were sequenced by Illumina’s mRNA-Seq PolyA selection and NuGEN ENCORE library preparation. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL11154 GPL10999
16 Samples
Download data: TXT
2.

Illumina HiSeq 2000 (Homo sapiens)

Platform
Accession:
GPL11154
ID:
100011154
3.

Illumina Genome Analyzer IIx (Homo sapiens)

Platform
Accession:
GPL10999
ID:
100010999
4.

BSO37_PolyA

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL10999
Series:
GSE47933
Download data
Sample
Accession:
GSM1162724
ID:
301162724
5.

BSO36_PolyA

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL10999
Series:
GSE47933
Download data
Sample
Accession:
GSM1162723
ID:
301162723
6.

BSO32N_PolyA

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL10999
Series:
GSE47933
Download data
Sample
Accession:
GSM1162722
ID:
301162722
7.

BSO30_PolyA

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL10999
Series:
GSE47933
Download data
Sample
Accession:
GSM1162721
ID:
301162721
8.

BSO29_PolyA

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL10999
Series:
GSE47933
Download data
Sample
Accession:
GSM1162720
ID:
301162720
9.

BSO28_PolyA

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL10999
Series:
GSE47933
Download data
Sample
Accession:
GSM1162719
ID:
301162719
10.

BSO19B_PolyA

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL10999
Series:
GSE47933
Download data
Sample
Accession:
GSM1162718
ID:
301162718
11.

DHF168_PolyA

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL10999
Series:
GSE47933
Download data
Sample
Accession:
GSM1162717
ID:
301162717
12.

BSO37_NuGEN

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL11154
Series:
GSE47933
Download data
Sample
Accession:
GSM1162732
ID:
301162732
13.

BSO36_NuGEN

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL11154
Series:
GSE47933
Download data
Sample
Accession:
GSM1162731
ID:
301162731
14.

BSO32N_NuGEN

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL11154
Series:
GSE47933
Download data
Sample
Accession:
GSM1162730
ID:
301162730
15.

BSO30_NuGEN

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL11154
Series:
GSE47933
Download data
Sample
Accession:
GSM1162729
ID:
301162729
16.

BSO29_NuGEN

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL11154
Series:
GSE47933
Download data
Sample
Accession:
GSM1162728
ID:
301162728
17.

BSO28_NuGEN

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL11154
Series:
GSE47933
Download data
Sample
Accession:
GSM1162727
ID:
301162727
18.

BSO19B_NuGEN

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL11154
Series:
GSE47933
Download data
Sample
Accession:
GSM1162726
ID:
301162726
19.

DHF168_NuGEN

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL11154
Series:
GSE47933
Download data
Sample
Accession:
GSM1162725
ID:
301162725
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