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Items: 3

1.

Impact of library preparation on downstream analysis and interpretation of RNA-seq data: comparison between Illumina PolyA and NuGEN Ovation protocol

(Submitter supplied) Objectives: The sequencing by the PolyA selection is the most common approach for library preparation. With limited amount or degraded RNA, alternative protocols such as the NuGEN have been developed. However, it is not yet clear how the different library preparations affect the downstream analyses of the broad applications of RNA sequencing. Methods and Materials: Eight human mammary epithelial cell (HMEC) lines with high quality RNA were sequenced by Illumina’s mRNA-Seq PolyA selection and NuGEN ENCORE library preparation. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL10999 GPL11154
16 Samples
Download data: TXT
2.

Illumina Genome Analyzer IIx (Homo sapiens)

Platform
Accession:
GPL10999
ID:
100010999
3.

BSO32N_PolyA

Organism:
Homo sapiens
Source name:
human mammary epithelial cell
Platform:
GPL10999
Series:
GSE47933
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Sample
Accession:
GSM1162722
ID:
301162722
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db=gds|term=GSM1162722[Accession]|query=1|qty=2|blobid=MCID_6724281cb9e6a62f8e6f5fd0|ismultiple=true|min_list=5|max_list=20|def_tree=20|def_list=|def_view=|url=/Taxonomy/backend/subset.cgi?|trace_url=/stat?
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