(Submitter supplied) Single-cell transcriptomics requires a method that is sensitive, accurate, and reproducible. Here, we present CEL-Seq2, a modified version of our CEL-Seq method, with three-fold higher sensitivity, lower costs, and less hands-on time. We also implemented CEL-Seq2 on Fluidigm’s C1 system, thereby providing its first single-cell, on-chip barcoding method, and detected gene expression changes accompanying the progression through the cell cycle in mouse fibroblast cells.
more...- Organism:
- Mus musculus; Caenorhabditis elegans
- Type:
- Expression profiling by high throughput sequencing
- Platforms:
- GPL17021 GPL18245
- 228 Samples
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