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Items: 3

1.

Embedding siRNA sequences targeting Apolipoprotein B100 in shRNA and miRNA scaffolds results in differential processing and in vivo efficacy

(Submitter supplied) Purpose: Identical predicted small interfering RNA (siRNA) sequences targeting Apolipoprotein B100 (siApoB) were embedded in shRNA (shApoB) or miRNA (miApoB) scaffolds and a direct compariso of the possible aspecific off-target effects in vivo was performed. Next generation sequencing (NGS) of small RNAs originating from shApoB- or miApoB-transfected cells revealed substantial differences in processing, resulting in different siApoB length, 5’ and 3’ cleavage sites and abundance of the guide or passenger strands. more...
Organism:
Homo sapiens; Mus musculus
Type:
Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing
Platforms:
GPL13112 GPL11154
7 Samples
Download data: PDF, TXT
Series
Accession:
GSE39187
ID:
200039187

PubMed Full text in PMC Similar studies SRA Run Selector

2.

Illumina HiSeq 2000 (Mus musculus)

Organism:
Mus musculus
8243 Series
187371 Samples
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Platform
Accession:
GPL13112
ID:
100013112

3.

miApoB

Organism:
Mus musculus
Source name:
miApoB
Platform:
GPL13112
Series:
GSE39187
Download data
Sample
Accession:
GSM957703
ID:
300957703

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db=gds|term=GSM957703[Accession]|query=1|qty=3|blobid=MCID_66643a4cbb845b085bd52a1d|ismultiple=true|min_list=5|max_list=20|def_tree=20|def_list=|def_view=|url=/Taxonomy/backend/subset.cgi?|trace_url=/stat?
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