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Links from GEO DataSets

Items: 16

1.

Transcriptome analysis of Leishmania infantum PNA+ and PNA- promastigotes by complete shotgun DNA microarrays

(Submitter supplied) Leishmania infantum is the causative agent of zoonotic visceral leishmaniasis in Mediterranean areas and also acts as an opportunistic parasite in HIV patients. Metacyclic promastigotes are transmitted during bloodmeals of the sand-fly host after development. Metacyclogenesis can be micmiked in axenic cultures and peanut lectin (PNA) agglutination followed by two-step centrifugation allows the separation of procyclic and metacyclic promastigotes in L. more...
Organism:
Leishmania infantum
Type:
Expression profiling by array
Platform:
GPL6781
3 Samples
Download data
Series
Accession:
GSE11269
ID:
200011269
2.

Comparative high-throughput gene expression analysis between cultured and sand fly vector-derived Leishmania infantum promastigotes.

(Submitter supplied) Leishmania infantum (Kinetoplastida:Trypanosomatidae) is the etiological agent of zoonotic visceral leishmaniasis in the Mediterranean basin. The motile promastigote stage infects the hematophagous sand fly vector host and amastigotes survives and multiplies within phagocytes of the mammalian host. Promastigotes are routinely cultured in liquid undefined media and are considered to mimic the environment within the sand fly gut. more...
Organism:
Leishmania infantum
Type:
Expression profiling by array
Platform:
GPL6781
1 Sample
Download data: TXT
Series
Accession:
GSE70992
ID:
200070992
3.

Whole-genome comparative RNA expression profiling of axenic and intracellular amastigote forms of Leishmania infantum

(Submitter supplied) Transcriptional analysis of L. infantum promastigote compared to L. infantum intracellular amastigote and transcriptional analysis of L. infantum promastigote compared to L. infantum axenic amastigote. The full-genome DNA microarrays includes one 70-oligonucleotides probe for each gene of L.infantum. Keywords: stage and culture condition
Organism:
Leishmania major; Leishmania infantum
Type:
Expression profiling by array
Platform:
GPL6855
10 Samples
Download data: GPR
Series
Accession:
GSE13983
ID:
200013983
4.

Interspecies expression profiling in Leishmania

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Leishmania major; Leishmania mexicana
Type:
Expression profiling by array
Platform:
GPL3505
11 Samples
Download data: TXT
Series
Accession:
GSE4444
ID:
200004444
5.

Expression profiling reveals differential gene expression Leishmania mexicana

(Submitter supplied) We examined the Leishmania mexicana transcriptome to identify differentially regulated mRNAs using high-density whole-genome oligonucleotide microarrays designed from the genome data of a closely related species, Leishmania major. Statistical analysis on array hybridization data representing 8156 predicted coding regions revealed 288 genes (3.5% of all genes) whose steady-state mRNA levels meet criteria for differential regulation between promastigotes and lesion-derived amastigotes. more...
Organism:
Leishmania mexicana; Leishmania major
Type:
Expression profiling by array
Platform:
GPL3505
9 Samples
Download data: TXT
Series
Accession:
GSE4431
ID:
200004431
6.

Interspecies hybridization versus intra-species hybridization for Leishmania promastigote RNA.

(Submitter supplied) We examined the Leishmania mexicana transcriptome to identify differentially regulated mRNAs using high-density whole-genome oligonucleotide microarrays designed from the genome data of a closely related species, Leishmania major. This experiment appears as Fig. 1 of the associated publication. Keywords: RNA expression profiling
Organism:
Leishmania mexicana; Leishmania major
Type:
Expression profiling by array
Platform:
GPL3505
4 Samples
Download data: TXT
Series
Accession:
GSE4430
ID:
200004430
7.

Leishmania major whole-genome 60-mer DNA oligonucleotide microarray

(Submitter supplied) This platform supports 11 60-mer DNA oligonucleotide probes synthesized twice on each slide for 8160 (98%) of the Leishmania major Friedlin genes annotated at www.genedb.org. Refer to: Timothy R. Holzer, W.R. McMaster, James D. Forney. “Expression profiling by whole-genome interspecies microarray hybridization reveals differential gene expression in procyclic promastigotes, lesion-derived amastigotes, and axenic amastigotes in Leishmania mexicana.” Molecular and Biochemical Parasitology 146 (2): 198-218. more...
Organism:
Leishmania major
3 Series
11 Samples
Download data
Platform
Accession:
GPL3505
ID:
100003505
8.

Comparison of cultured and sand fly-derived promastigotes by means of RNA-Seq

(Submitter supplied) A laboratory colony of Phlebotomus perniciosus sand flies was maintained. Sand flies were infected with cultured Leishmania infantum promastigotes in stationary phase. Ten infected sand flies were dissected after 5 days and promastigotes within the gut pooled. The cells were immediately washed in PBS once and lysed in TRIzol reagent (Life Technologies). RNA isolation was completed according to the manufacturer's instructions, obtaining 63ng. more...
Organism:
Leishmania infantum
Type:
Expression profiling by high throughput sequencing
Platform:
GPL23911
2 Samples
Download data: XLSX
Series
Accession:
GSE102668
ID:
200102668
9.

Transcriptional analysis of wild-type Leishmania species between both stages using full-genome DNA microarrays

(Submitter supplied) Transcriptional analyses of L. infantum promastigote compared to L. infantum intracellular amastigote, and L. major promastigote compared to L. major intracellular amastigote The full-genome DNA microarray includes one 70mer-oligonucleotide probe for each gene of L. infantum and for each gene of L.major LV39 Keywords: stage-specific comparison
Organism:
Leishmania major; Leishmania infantum
Type:
Expression profiling by array
Platform:
GPL6855
10 Samples
Download data: GPR
Series
Accession:
GSE10407
ID:
200010407
10.

Application of Serial Analysis of Gene Expression to the study of the gene expression profile of Leishmania infantum chagasi promastigote

(Submitter supplied) This study describes the application of the LongSAGE methodology to study the gene expression profile in Leishmania infantum chagasi. A tag library was created using the LongSAGE method and consisted of 14,208 tags of 17 bases. Of these, 8,427 (59.3%) were distinct. BLAST research of the 1,645 most abundant tags showed that 12.8% of them identified the coding sequences of genes, while 82% (1,349/1,645) identified one or more genomic sequences that did not correspond with open reading frames. more...
Organism:
Leishmania chagasi
Type:
Expression profiling by SAGE
Platform:
GPL11414
1 Sample
Download data
Series
Accession:
GSE29369
ID:
200029369
11.

Expression data for stress treatment in rice seedlings

(Submitter supplied) Drought, salinity and sub-optimal temperatures are stresses that cause adverse effects on the growth of plants and the productivity of crops. In this study, we have analyzed the expression profiles of rice genes under control and abiotic stress conditions using microarray technology to identify the genes differentially expressed during various abiotic stresses. Keywords: Stress treatment
Organism:
Oryza sativa
Type:
Expression profiling by array
Platform:
GPL2025
12 Samples
Download data: CEL
Series
Accession:
GSE6901
ID:
200006901
12.

Expression data for reproductive development in rice

(Submitter supplied) Plants initially undergo a period of vegetative development, in which it produces leaves from shoot apical meristem (SAM) and roots from the root apical meristem. Later in development, the SAM undergoes a change in fate and enters reproductive development called as floral transition, producing flowers and seeds. Our understanding of the molecular and genetic mechanisms that underlie reproductive development in plants has increased tremendously in the past decade, essentially through the work on Arabidopsis. more...
Organism:
Oryza sativa
Type:
Expression profiling by array
Platform:
GPL2025
45 Samples
Download data: CEL
Series
Accession:
GSE6893
ID:
200006893
13.

Rice seedling hormone treatment

(Submitter supplied) Indica rice seedlings of IR64 variety were grown hydroponically for 7-days in a culture room with a daily photoperiodic cycle of 14h light and 10h dark. Seedlings were incubated in 0.1% dimethyl sulfoxide (control) or 50 micromolar solutions of indole-3-acetic acid (IAA treatment) and benzyl aminopurine (BAP treatment) for 1h and 3h. Equal amounts of 1h and 3h samoles were pooled for each treatment before RNA isolation. more...
Organism:
Oryza sativa
Type:
Expression profiling by array
Platform:
GPL2025
6 Samples
Download data: CAB, CEL, EXP
Series
Accession:
GSE5167
ID:
200005167
14.

Within and between-species transcript profiling during cotton fibre development

(Submitter supplied) A gene expression profiling study on two major cotton species that are cultivated for fibre, Gossypium hirsutum (L.) and Gossypium barbadense (L.), at different stages during fibre development using a printed cDNA microarray was undertaken to identify potential candidate genes for manipulation to improve fibre quality. Keywords: Species comparison, development
Organism:
Gossypium barbadense; Gossypium hirsutum
Type:
Expression profiling by array
Platform:
GPL4043
18 Samples
Download data: GPR
Series
Accession:
GSE10363
ID:
200010363
15.

Adaptive differences in gene expression associated with stress tolerance in the soil arthropod Orchesella cincta

(Submitter supplied) Field-selected tolerance to heavy metals has been reported for Orchesella cincta (Arthropoda: Collembola) populations occurring at metal-contaminated mining sites. This tolerance is correlated with heritable increase of metal excretion efficiency; less pronounce cadmium induced growth reduction and, over-expression of the metallothionein gene. We applied transcriptomics to determine differential gene expression caused by this abiotic stress in reference and cadmium tolerant populations. more...
Organism:
Orchesella cincta
Type:
Expression profiling by array
Platform:
GPL7876
16 Samples
Download data: TXT
Series
Accession:
GSE14076
ID:
200014076
16.

Differential gene expression in DEVH1 knock-in Leishmania infantum promastigotes

(Submitter supplied) The DEAD/H RNA helicase LINF_220021200 (DEVH1) gene from Leishmania infantum (Kinetoplastida:Trypanosomatidae) was cloned in the pTEX expression plasmid vector for trypanosomatids. Leishmania infantunm promastigotes were transfected and a knock-in L. infantum promastigote cell line was selected with geneticin (G418). A pTEX control promastigote line was also generated. Then, three independent biological replicate cultures of each pTEX-DEVH1 and pTEX promastigote lines were performed in the presence of the selective agent. more...
Organism:
Leishmania infantum
Type:
Expression profiling by array
Platform:
GPL6781
1 Sample
Download data: GPR, TXT
Series
Accession:
GSE202674
ID:
200202674
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