GEO DataSets

(Submitter supplied) Serial Analysis of Gene Expression (LongSAGE) was applied for gene expression profiling in seeds of oilseed rape (Brassica napus ssp. napus). The usefulness of this technique for detailed expression profiling in a non-model organism was demonstrated for the highly complex, neither fully sequenced nor annotated genome of B. napus by applying a tag-to-gene matching strategy based on Brassica ESTs and the annotated proteome of the closely related model crucifer A. more...

Organism:

Brassica napus

Type:

Expression profiling by SAGE

Platform:

GPL8033

2 Samples

Download data

(Submitter supplied) Seed coat colour is determined by the type of pigment deposited in the seed coat cells. It is related to important agronomic traits of seeds, such as seed dormancy, longevity, oil content, protein content and fibre content. In Brassica napus, inheritance of seed coat colour is related to maternal effects and pollen effects (xenia effects). In this research, we isolated a mutation of yellow seeded B. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15579

2 Samples

Download data: FASTA, XLS

(Submitter supplied) In species with exalbuminous seeds such as crucifer oilseeds and legumes, the endosperm is eventually consumed and its space occupied by the embryo during seed development. However, the main constituent of the early developing seed is the liquid endosperm, and most of the carbon resources for the ensuing stages of seed development arrive at the embryo through the endosperm. In contrast to the extensive study of species with persistent endosperm, little is known about the global gene expression pattern in the endosperm of exalbuminous seed species. more...

Organism:

Brassica napus

Type:

Expression profiling by array

Platform:

GPL8090

24 Samples

Download data: GPR

(Submitter supplied) A set of 10,642 PCR primer pairs were designed and corresponding amplicons were produced for preparation of a targeted seed cDNA microarray. cDNA amplicons were printed on Corning UltraGAPS slides with the Chipwriter Pro microarray printer (Bio-Rad). The microarray consists of 12 row × 4 column sub-arrays, and each sub-array has 23 row × 22 column (506) spots. Spots were printed in duplicate side-by-side by columns, and the total number of spots on the microarray is 24288, of which 22,656 spots are cDNA probes. more...

Organism:

Brassica napus

1 Series

24 Samples

Download data: GAL

(Submitter supplied) Time course of gene expression profiles during seed development and maturation in Brassica napus were studied using Combimatrix Brassica microarray.

Organism:

Brassica napus

Type:

Expression profiling by array

Platform:

GPL15260

48 Samples

Download data: GPR

(Submitter supplied) Transcription profiling of Brassica rapa, Brassica oleracea and Brassica napus I and II The nuclear genomes of the resynthesised B. napus lines should be identical but, as one (B. napus I) involved a cross of B. oleracea onto B. rapa, and the other (B. napus II) involved a cross of B rapa onto B. oleracea, they differ in cytoplasm, and hence contain different chloroplast and mitochondrial genomes.

Organism:

Brassica napus; Brassica rapa; Brassica; Brassica oleracea

Type:

Expression profiling by array

Platform:

GPL8513

16 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Brassica napus

Type:

Non-coding RNA profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:

GPL15579

4 Samples

Download data: TXT

(Submitter supplied) MicroRNAs (miRNAs) are important post-transcriptional regulators of plant development and seed formation. In Brassica napus, an important edible oil crop, valuable lipids are synthesized and stored in specific seed tissues during embryogenesis. The miRNA transcriptome of B. napus is currently poorly characterized, especially at different seed developmental stages. This work aims to describe the miRNAome of developing seeds of B. more...

Organism:

Brassica napus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15579

2 Samples

Download data: TXT

(Submitter supplied) MicroRNAs (miRNAs) are important post-transcriptional regulators of plant development and seed formation. In Brassica napus, an important edible oil crop, valuable lipids are synthesized and stored in specific seed tissues during embryogenesis. The miRNA transcriptome of B. napus is currently poorly characterized, especially at different seed developmental stages. This work aims to describe the miRNAome of developing seeds of B. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15579

2 Samples

Download data: TXT

(Submitter supplied) Identification of differentially expressed genes in seeds and silique walls at the seed-filling stage in Brassica napus through transcriptional profiling

Organism:

Brassica; Brassica napus

Type:

Expression profiling by array

Platform:

GPL8513

7 Samples

Download data: LSR

(Submitter supplied) The gynoecium is one of the most complex organs of angiosperms specialized for seed production and dispersal, but only several genes important for ovule or embryo sac development were identified by using female sterile mutants. The female sterility in oilseed rape (Brassica napus) was before found to be related with one alien chromosome from another crucifer Orychophragmus violaceus. Herein, the developmental anatomy and comparative transcript profiling (RNA-seq) for the female sterility were performed to reveal the genes and possible metabolic pathways behind the formation of the damaged gynoecium. more...

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15579

2 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Methylation profiling by high throughput sequencing

Platform:

GPL29911

88 Samples

Download data: TXT

(Submitter supplied) To investigate the expression and methylation patterns of hybrid embryo development in Brassica napus, we performed pattern analysis on different developmental stages of parental and hybrid embryos

Organism:

Brassica napus

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL29911

22 Samples

Download data: TXT

(Submitter supplied) To investigate the expression and methylation patterns of hybrid embryo development in Brassica napus, we performed pattern analysis on different developmental stages of parental and hybrid embryos

Organism:

Brassica napus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL29911

33 Samples

Download data: TXT

(Submitter supplied) To investigate the expression and methylation patterns of hybrid embryo development in Brassica napus, we performed pattern analysis on different developmental stages of parental and hybrid embryos

Organism:

Brassica napus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29911

33 Samples

Download data: TXT

(Submitter supplied) The fungal pathogen Sclerotinia sclerotiorum infects a broad range of dicotyledonous plant species and is the causative agent of stem rot in Brassica napus. To elucidate the mechanisms underlying the defense response, we studied the patterns of gene expression in a partially resistant variety of ZhongYou 821 (ZY821) and a susceptible line from Westar over five time points, 6, 12, 24, 48, and 72 hours post-inoculation (hpi) using a B. more...

Organism:

Brassica napus

Type:

Expression profiling by array

Platform:

GPL7499

60 Samples

Download data: TXT

(Submitter supplied) MicroRNAs and siRNAs are important regulators of plant development and seed formation, yet their population and abundance in the oil crop Brassica napus are still less understood, especially at different developmental stages and among cultivars with varied seed oil contents. Here, we systematically analyzed the small RNA expression profiles of Brassica napus seeds at early embryonic developmental stages in a high oil content and a low oil content Brassica napus cultivars, both cultured in two environments. more...

Organism:

Brassica napus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15059

4 Samples

Download data: TXT

(Submitter supplied) The effect of the number of pods on the main inflorescence (NPMIs) on seed yield in Brassica napus plants grown at high density is a topic of great economic and scientific interest. We sought to identify patterns of gene expression that are associated with inflorescence and PMI differentiation and development in Brassica napus.

Organism:

Brassica oleracea; Brassica rapa; Brassica napus

Type:

Expression profiling by array

Platform:

GPL18717

12 Samples

Download data: TXT

(Submitter supplied) We anlalyzed gene expession using an Arabidopsis 26K 70mer spotted microarray Differential expression was tested using two ANOVA models: 1) a per-gene variance ANOVA, and 2) a common variance ANOVA

Organism:

Brassica oleracea; Arabidopsis thaliana; Brassica napus; Brassica sp.; Brassica rapa

Type:

Expression profiling by array

Platform:

GPL7536

7 Samples

Download data: GPR

(Submitter supplied) Identification of nutrient-specific microRNA expressed in Arabidopsis seedlings

Organism:

Arabidopsis thaliana

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL9302

5 Samples

Download data