GEO DataSets

(Submitter supplied) We performed a targeted proteomics analysis of Cutaneous Lupus Erythematosus (CLE) patient blister biopsies and plasma using Olink Proteomics panels to validate the blister biopsy technique using previously identified biomarkers, to confirm protein-level expression of biomarkers previously identified at the RNA level, and to identify new biomarkers of disease.

Organism:

Homo sapiens

Type:

Other

Platform:

GPL30526

38 Samples

Download data: CSV

(Submitter supplied) We performed spatial transcriptomics on a case series of different clinical subtypes of cutaneous lupus erythematosus including acute cutaneous lupus erythematosus (malar rash, systemic lupus erythematosus). Our goals were to (1) determine which differentially expressed genes (DEGs) could be attributed to specific cell populations in specific locations within the tissue, (2) determine if spatial transcriptomics could better distinguish between CLE clinical subtypes than bulk RNA approaches and (3) examine potential cell-cell communication pathways within the skin lesions.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL24676

41 Samples

Download data: DCC, PKC, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Other

Platforms:

GPL30526 GPL30525

76 Samples

Download data

(Submitter supplied) We performed a targeted proteomics analysis of Cutaneous Lupus Erythematosus (CLE) patient blister biopsies and plasma using Olink Proteomics panels to validate the blister biopsy technique using previously identified biomarkers, to confirm protein-level expression of biomarkers previously identified at the RNA level, and to identify new biomarkers of disease.

Organism:

Homo sapiens

Type:

Other

Platform:

GPL30525

38 Samples

Download data: TXT

(Submitter supplied) We performed a comparative immunology case study of client-owned dogs to determine if immune and skin gene expression profiles in spontaneous canine chronic cutaneous lupus erythematosus (CCLE) with concomitant systemic autoimmunity mirror those observed in human CCLE.

Organism:

Canis lupus familiaris

Type:

Expression profiling by array

Platform:

GPL28844

9 Samples

Download data: RCC

(Submitter supplied) We performed a comparative immunology case study of client-owned dogs to determine if immune and skin gene expression profiles in spontaneous canine pemphigus mirror those observed in human pemphigus

Organism:

Canis lupus familiaris

Type:

Other

Platform:

GPL28844

9 Samples

Download data: RCC, TXT

(Submitter supplied) We performed a comparative immunology case study of client-owned dogs to determine if immune and skin gene expression profiles in spontaneous canine cutaneous lupus erythematosus mirror those observed in human cutaneous lupus.

Organism:

Canis lupus familiaris

Type:

Expression profiling by array

Platform:

GPL28844

15 Samples

Download data: RCC

(Submitter supplied) Cutaneous lupus erythematosus (CLE) is a disfiguring disease that can exist as an independent entity or as a manifestation of systemic lupus erythematosus (SLE) where up to 70% of patients experience lesions during their disease course. Subacute CLE (sCLE) is an inflammatory lesion with associated erythema in papulosquamous or annular formations. Typically, sCLE does not scar but depigmentation can occur. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL19983

103 Samples

Download data: CEL, TXT

(Submitter supplied) The microarray experiment was employed to evaluate the gene expressions in cutaneous lupus

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17692

72 Samples

Download data: CEL

(Submitter supplied) Cutaneous lupus erythematosus (CLE) is a photosensitive autoimmune disease characterized by a strong type-I-interferon (IFN) associated inflammation. Keratinocytes are known to determine the interface-dermatitis-pattern in CLE by production of proinflammatory cytokines in the lower epidermis. These cytokines drive a cytotoxic anti-epithelial immune response resulting in keratinocytic cell death and release of endogenous nucleic acids (eNA). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16699

16 Samples

Download data: TXT

(Submitter supplied) SLE patients exhibit increased interferon signatures in their skin secondary to increased production and a robust, skewed IFN response that is regulated by PITX1. Targeting of these exaggerated pathways may prove to be beneficial to prevent and treat hyperinflammatory responses in SLE skin.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20301

72 Samples

Download data: TXT

(Submitter supplied) Background: Atopic dermatitis (AD) is the most common chronic inflammatory skin disease, but its complex pathogenesis is only insufficiently understood, resulting in still limited treatment options. Objective: We sought to characterize AD on both transcriptomic and proteomic levels in humans. Methods: We used skin suction blistering, a painless and nonscarring procedure that can simultaneously sample skin cells and interstitial fluid. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21290

15 Samples

Download data: MTX, TSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array; Protein profiling by protein array

Platforms:

GPL30820 GPL30876

28 Samples

Download data: RCC

(Submitter supplied) We performed targeted protein expression analysis on cultured Th0, Th1 and Th2 cells, and on T cells treated with known drug triggers of cutaneous lupus erythematosus (CLE). In tandem, we performed targeted protein expression analysis on serum from first vs flare Th2-injected mice in the CLE model.

Organism:

Mus musculus

Type:

Protein profiling by protein array

Platform:

GPL30876

16 Samples

Download data: XLSX

(Submitter supplied) We performed gene expression analysis on cultured Th0, Th1 and Th2 cells pre-injection, and on enriched T cells from lesional skin of cutaneous lupus erythematosus (CLE) mice post-injection, to compare differences in transcription that could predict skin tropism and flare

Organism:

Mus musculus

Type:

Other

Platform:

GPL30820

12 Samples

Download data: RCC, XLSX