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Status |
Public on Apr 30, 2018 |
Title |
Single-cell multi-omics sequencing of human early embryos |
Organism |
Homo sapiens |
Experiment type |
Methylation profiling by high throughput sequencing
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Summary |
DNA methylation, chromatin states, and their interrelationships represent critical epigenetic information, but those are largely unknown in human early embryos. Here, we apply scCOOL-seq (Chromatin Overall Omic-scale Landscape Sequencing) to generate a genome-wide map of DNA methylation and chromatin accessibility at single-cell resolution during human preimplantation development. Unlike in mice, the chromatin of paternal genome is already more open than that of maternal genome at the mid-zygote stage in humans, and this state is maintained until the 4-cell stage. After fertilization, genes with high variations in DNA methylation and those with high variations in chromatin accessibility tend to be two different sets. Furthermore, 1,797 (35%) out of 5,155 widely open chromatin regions in promoters closed when transcription activity was inhibited, indicating a feedback mechanism between transcription and open chromatin maintenance. Our work paves the way for dissecting the complex, yet highly coordinated, epigenetic reprogramming during human preimplantation development.
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Overall design |
In total, we analyzed 265 single cells (13 sperm, 9 oocytes, 10 zygotes, 14 2-cell blastomeres, 26 4-cell blastomeres, 48 8-cell blastomeres, 25 morulae blastomeres, 50 ICM, 48 TE and 22 hESCs) as well as 23 alpha-Amanitin treated 8-cell blastomeres.
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Contributor(s) |
Li L, Fan G, Gao Y, Tang F |
Citation(s) |
29915357 |
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Submission date |
Jun 20, 2017 |
Last update date |
Jul 25, 2021 |
Contact name |
Lin Li |
E-mail(s) |
lilin2019@i.smu.edu.cn
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Organization name |
Southern Medical University
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Department |
Pathophysiology
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Street address |
Shatai South Road
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City |
Guangzhou |
ZIP/Postal code |
510515 |
Country |
China |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (288)
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Relations |
BioProject |
PRJNA391189 |
SRA |
SRP109843 |
Supplementary file |
Size |
Download |
File type/resource |
GSE100272_RAW.tar |
51.3 Gb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
Processed data provided as supplementary file |
Raw data are available in SRA |
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