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Series GSE100272 Query DataSets for GSE100272
Status Public on Apr 30, 2018
Title Single-cell multi-omics sequencing of human early embryos
Organism Homo sapiens
Experiment type Methylation profiling by high throughput sequencing
Summary DNA methylation, chromatin states, and their interrelationships represent critical epigenetic information, but those are largely unknown in human early embryos. Here, we apply scCOOL-seq (Chromatin Overall Omic-scale Landscape Sequencing) to generate a genome-wide map of DNA methylation and chromatin accessibility at single-cell resolution during human preimplantation development. Unlike in mice, the chromatin of paternal genome is already more open than that of maternal genome at the mid-zygote stage in humans, and this state is maintained until the 4-cell stage. After fertilization, genes with high variations in DNA methylation and those with high variations in chromatin accessibility tend to be two different sets. Furthermore, 1,797 (35%) out of 5,155 widely open chromatin regions in promoters closed when transcription activity was inhibited, indicating a feedback mechanism between transcription and open chromatin maintenance. Our work paves the way for dissecting the complex, yet highly coordinated, epigenetic reprogramming during human preimplantation development.
 
Overall design In total, we analyzed 265 single cells (13 sperm, 9 oocytes, 10 zygotes, 14 2-cell blastomeres, 26 4-cell blastomeres, 48 8-cell blastomeres, 25 morulae blastomeres, 50 ICM, 48 TE and 22 hESCs) as well as 23 alpha-Amanitin treated 8-cell blastomeres.
 
Contributor(s) Li L, Fan G, Gao Y, Tang F
Citation(s) 29915357
Submission date Jun 20, 2017
Last update date Jul 25, 2021
Contact name Lin Li
E-mail(s) lilin2019@i.smu.edu.cn
Organization name Southern Medical University
Department Pathophysiology
Street address Shatai South Road
City Guangzhou
ZIP/Postal code 510515
Country China
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (288)
GSM2676907 Sperm_1
GSM2676908 Sperm_2
GSM2676909 Sperm_3
Relations
BioProject PRJNA391189
SRA SRP109843

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100272_RAW.tar 51.3 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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