NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE100303 Query DataSets for GSE100303
Status Public on Dec 31, 2024
Title Histone demethylation and c-MYC activation enhance translational capacity in response to amino acid restriction [RNA-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Nutrient limitation may elicit adaptive epigenetic changes but the nature and mechanisms of the cellular response to specific nutrient deficiencies are incompletely understood. We report that depriving human cells of amino acids (AAs) induces specific loss of H4K20me1 from gene bodies and elevated binding of c-MYC at promoters genome-wide. These effects are most pronounced at ribosomal protein and translation initiation genes, which are upregulated, leading to enhanced protein synthetic capacity. Combination of H4K20 methyltransferase depletion and c-MYC over-expression in rich media is required and sufficient to recapitulate the effects of AA restriction. Our data reveal an unexpected and epigenetically implemented increase in translational capacity when AAs are limiting, likely to safeguard the proteome by making effective use of limited resources.
 
Overall design Examine the expression of cells cultured in complete DMEM medium or in DMEM lacking amino acids by RNA-seq analysis. Examine the expression of cells cultured in complete DMEM medium with Setd8 knockdown and/or c-Myc overexpression.
 
Contributor(s) Cheng C, Kurdistani SK
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jun 21, 2017
Last update date Dec 31, 2024
Contact name Siavash K Kurdistani
E-mail(s) skurdistani@mednet.ucla.edu
Organization name UCLA
Department Biological Chemistry
Lab Kurdistani
Street address 615 Charles E Young Dr South
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (14)
GSM2677623 HeLa_AA_mRNAseq_biological-replicate1
GSM2677624 HeLa_NOAA_mRNAseq_biological-replicate1
GSM2677625 HeLa_AA_controlKD_mRNAseq
This SubSeries is part of SuperSeries:
GSE100304 Histone demethylation and MYC activation enhance translational capacity in response to amino acid restriction
Relations
BioProject PRJNA391298
SRA SRP110024

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100303_RAW.tar 109.5 Mb (http)(custom) TAR (of WIG)
GSE100303_RPKM_RNAseq.xlsx 9.9 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap