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Status |
Public on Dec 31, 2024 |
Title |
Histone demethylation and c-MYC activation enhance translational capacity in response to amino acid restriction [RNA-seq] |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Nutrient limitation may elicit adaptive epigenetic changes but the nature and mechanisms of the cellular response to specific nutrient deficiencies are incompletely understood. We report that depriving human cells of amino acids (AAs) induces specific loss of H4K20me1 from gene bodies and elevated binding of c-MYC at promoters genome-wide. These effects are most pronounced at ribosomal protein and translation initiation genes, which are upregulated, leading to enhanced protein synthetic capacity. Combination of H4K20 methyltransferase depletion and c-MYC over-expression in rich media is required and sufficient to recapitulate the effects of AA restriction. Our data reveal an unexpected and epigenetically implemented increase in translational capacity when AAs are limiting, likely to safeguard the proteome by making effective use of limited resources.
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Overall design |
Examine the expression of cells cultured in complete DMEM medium or in DMEM lacking amino acids by RNA-seq analysis. Examine the expression of cells cultured in complete DMEM medium with Setd8 knockdown and/or c-Myc overexpression.
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Contributor(s) |
Cheng C, Kurdistani SK |
Citation missing |
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Submission date |
Jun 21, 2017 |
Last update date |
Dec 31, 2024 |
Contact name |
Siavash K Kurdistani |
E-mail(s) |
skurdistani@mednet.ucla.edu
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Organization name |
UCLA
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Department |
Biological Chemistry
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Lab |
Kurdistani
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Street address |
615 Charles E Young Dr South
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City |
Los Angeles |
State/province |
CA |
ZIP/Postal code |
90095 |
Country |
USA |
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Platforms (2) |
GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (14)
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This SubSeries is part of SuperSeries: |
GSE100304 |
Histone demethylation and MYC activation enhance translational capacity in response to amino acid restriction |
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Relations |
BioProject |
PRJNA391298 |
SRA |
SRP110024 |