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Series GSE100359 Query DataSets for GSE100359
Status Public on Sep 15, 2017
Title Integrated Transcriptomic and Proteomic Dynamics of Everolimus Treatment in B Lymphoblastoid Cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The investigation aims to profile the molecular dynamics of Everolimus treatment in cells. Specifically, a B-lymphoblast cells line was treated with Everolimus and sampled every half-hour over a 12 hour period. Treated and untreated cells were processed to extract RNA and protein that were subsequently used in high-throughput analysis of the transcriptome (RNA-Sequencing) and proteome (mass spectrometry).
 
Overall design Examination of dynamics in a treated cell line and a parallel untreated controlline, both sampled over 12hrs every half hour. Validation using a new repeated experiment, with treated and untreated tracks, sampled over 6 selected time points.
 
Contributor(s) Mias GI, Brooks LK
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NIH grant(s)
Grant ID Grant title Affiliation Name
R00 HG007065 Integrative Dynamic Omics Profiling: A First Step Towards Personalized Medicine MICHIGAN STATE UNIVERSITY George Ioannou Mias
Submission date Jun 22, 2017
Last update date Jul 25, 2021
Contact name George Mias
Organization name Michigan State University
Department Biochemistry and Molecular Biology
Street address 775 Woodlot Drive, Rm1319
City East Lansing
ZIP/Postal code 48824
Country USA
 
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (60)
GSM2679452 Untreated Timepoint 0
GSM2679453 Untreated Timepoint 1
GSM2679454 Untreated Timepoint 2
Relations
BioProject PRJNA391449
SRA SRP110148

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE100359_RAW.tar 153.5 Mb (http)(custom) TAR (of FPKM_TRACKING)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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