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Status |
Public on Sep 16, 2017 |
Title |
CD95L derived si- and shRNAs and the CD95L mRNA kill cancer cells through an RNAi mechanism by targeting survival genes [siL3.RNAseq.lg] |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We provide evidence that shRNAs and siRNAs derived from CD95 and CD95L preferentially target the 3' UTRs of survival genes culminating in a very robust mode of cell death we call DISE (Death Induced by Survival gene Elimination)
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Overall design |
RNA isolated from HeyA8 cells 48 hrs after transfection with either a nontargeting siRNA (siScr) or a CD95L derived siRNAs (siL3) were subjected to deep sequencing, using Illumina HiSeq4000.
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Contributor(s) |
Putzbach W, Peter ME, Bartom E |
Citation(s) |
29063830 |
NIH grant(s) |
Grant ID |
Grant title |
Affiliation |
Name |
R35 CA197450 |
DISE - a natural cancer surveillance mechanism - a new road to cancer therapy |
NORTHWESTERN UNIVERSITY AT CHICAGO |
Marcus E. Peter |
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Submission date |
Jul 11, 2017 |
Last update date |
Sep 08, 2022 |
Contact name |
Marcus Peter |
E-mail(s) |
m-peter@northwestern.edu
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Organization name |
Northwestern University Feinberg School of Medicine
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Street address |
303 East Superior Street, Lurie 6-123
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City |
Chicago |
State/province |
IL |
ZIP/Postal code |
60611 |
Country |
USA |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (8)
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This SubSeries is part of SuperSeries: |
GSE87817 |
CD95L derived si- and shRNAs kill cancer cells through an RNAi mechanism by targeting survival genes |
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Relations |
BioProject |
PRJNA393831 |
SRA |
SRP111532 |