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Series GSE101525 Query DataSets for GSE101525
Status Public on Jul 17, 2018
Title Overall expression of genes in CRYM overexpressed Prostate cancer cell line LNCaP
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary RNA sequencing was employed to profile the entire mRNA transcripts. Total RNA from LNCaP transfected with control vector or CRYM bearing vector was isolated. RNA and samples were subjected to the sequencing on illumina's HiSeq 4000 platform. RNA-seq revealed the expression of 58,540 transcripts and of 34,878 (59.6 %) genes were expressed in LNCaP cells. 3226 (9.25 %) genes were altered by CRYM and 2.72 % were upregulated and 6.53 % were downregulated.
 
Overall design Cells were in transiently transfected cells with CRYM vector vs control in duplicate. GFP expression was monitored and total RNA was isolated at 48 hours upon transfection.
 
Contributor(s) Aksoy O, Merkel O
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Submission date Jul 17, 2017
Last update date Jul 25, 2021
Contact name Olaf Merkel
E-mail(s) olaf.merkel@meduniwien.ac.at
Organization name Medical University of Vienna
Department Clinical Institute of Pathology
Lab Kenner Laboratory
Street address Waehringer Guertel 18-20
City Vienna
ZIP/Postal code 1090
Country Austria
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (4)
GSM2705506 LNCaP control 1
GSM2705507 LNCaP control 2
GSM2705508 LNCaP CRYM 1
Relations
BioProject PRJNA394730
SRA SRP112571

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE101525_RAW.tar 10.4 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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