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Status |
Public on Feb 14, 2018 |
Title |
Transcriptome-wide analysis of the role of HTLV-1 Tax PBM in T-Cells from infected humanized-mice (hu-Mice) |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Human T-lymphotropic virus type 1 (HTLV-1) is associated with the development of Adult T-cell Leukemia, an aggressive CD4+ T-cells malignancy. Here, we have developed a new procedure to infect humanized mice with proviruses displaying specific mutations, such as one leading to the loss of the PDZ domain-binding motif (PBM) of Tax. In order to specifically analyze the in vivo role of the PBM of Tax, a comparative study of infected hu-mice was performed. We used next-generation sequencing to perform genome-wide transcriptomic analysis of T-cells infected with wild-type HTLV-1 virus or with virus bearing a mutated form of Tax lacking the PBM. Our results suggest that Tax PBM might be involved in the regulation of genes implicated in proliferation, apoptosis and cytoskeleton organization.
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Overall design |
mRNA profiles of T-cells obtained from hu-Mice infected with wild-type or Tax-PBM HTLV-1 were generated by deep-sequencing in triplicates using Illumina’s Hiseq3000 platform.
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Contributor(s) |
Ricci EP, Duc-Dodon M, Perès E, Blin J |
Citation(s) |
29566098 |
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Submission date |
Aug 03, 2017 |
Last update date |
Jul 25, 2021 |
Contact name |
Emiliano P Ricci |
E-mail(s) |
emiliano.ricci@ens-lyon.fr
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Organization name |
INSERM
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Department |
Centre International de Recherche en Infectiologie
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Street address |
46 allée d'Italie
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City |
Lyon |
ZIP/Postal code |
69007 |
Country |
France |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (6)
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Relations |
BioProject |
PRJNA397039 |
SRA |
SRP114773 |