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| Status |
Public on Oct 15, 2019 |
| Title |
RNA sequencing to identify NPR-8-regulated genes in C. elegans defense against pathogen infection |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To gain molecular insights on how NPR-8 regulates C. elegans defense against pathogen infection, we used RNA sequencing to profile gene expression in npr-8(ok1439) animals relative to wild-type animals with or without P. aeruginosa infection. We found that NPR-8 suppresses the expression of genes related to cuticle structure activity including collagen genes, and lack of NPR-8-mediated suppression in npr-8(ok1439) animals contributes to their improved survival against P. aeruginosa infection.
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| Overall design |
Five replicates of four groups of RNA samples (npr-8(ok1439) and wild-type animals with or without exposure to P. aeruginosa for 24 hrs) were sequenced. Gene expression was compared between groups to identify NPR-8-regulated genes under uninfection or infection conditions.
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| Contributor(s) |
Liu Y, Sellegounder D, Sun J |
| Citation(s) |
31799388 |
| |
| Submission date |
Nov 14, 2018 |
| Last update date |
Mar 03, 2022 |
| Contact name |
Yiyong Liu |
| Organization name |
Washington State University
|
| Street address |
412 E Spokane Falls Blvd
|
| City |
Spokane |
| State/province |
WA |
| ZIP/Postal code |
99202 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA505517 |
| SRA |
SRP168626 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE122544_N2OP50_vs_N2PA14.deseq.counts.csv.gz |
460.8 Kb |
(ftp)(http) |
CSV |
| GSE122544_N2OP50_vs_N2PA14.deseq.results.csv.gz |
1.3 Mb |
(ftp)(http) |
CSV |
| GSE122544_N2OP50_vs_N2PA14.deseq.results.significant.csv.gz |
548.0 Kb |
(ftp)(http) |
CSV |
| GSE122544_N2OP50_vs_npr8OP50.deseq.counts.csv.gz |
461.4 Kb |
(ftp)(http) |
CSV |
| GSE122544_N2OP50_vs_npr8OP50.deseq.results.csv.gz |
1.3 Mb |
(ftp)(http) |
CSV |
| GSE122544_N2OP50_vs_npr8OP50.deseq.results.significant.csv.gz |
557.3 Kb |
(ftp)(http) |
CSV |
| GSE122544_N2PA14_vs_npr8PA14.deseq.counts.csv.gz |
471.1 Kb |
(ftp)(http) |
CSV |
| GSE122544_N2PA14_vs_npr8PA14.deseq.results.csv.gz |
1.3 Mb |
(ftp)(http) |
CSV |
| GSE122544_N2PA14_vs_npr8PA14.deseq.results.significant.csv.gz |
576.2 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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