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| Status |
Public on Apr 03, 2020 |
| Title |
Endogenous siRNAs promote proteostasis and longevity in germline-less Caenorhabditis elegans |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
How lifespan and the rate of aging are set is a key problem in biology. Small RNAs are conserved molecules that impact diverse biological processes through the control of gene expression. However, in contrast to miRNAs, the role of endo-siRNAs in aging remains unexplored. Here, by combining deep sequencing and genomic and genetic approaches in C.CaenorhabditisC. elegans elegans, we reveal an unprecedented role for endo-siRNA molecules in the maintenance of proteostasis and lifespan extension in germline-less animals. Furthermore, we identify an endo-siRNA-regulated tyrosine phosphatase, which limits the longevity of germline-less animals by restricting the activity of the heat shock transcription factor HSF-1. Altogether, our findings point to endo-siRNAs as a link between germline removal and the HSF-1 proteostasis and longevity-promoting somatic pathway. This establishes a role for endo siRNAs in the aging process and identifies downstream genes and physiological processes that are regulated by the endo siRNAs to affect longevity.
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| Overall design |
glp-1(e2144) and dcr-1(mg375) glp-1(e2144) C. elegans were raised at 25 degrees until day 1 of adulthood. Low molecular RNA fraction was extracted using miRVana miRNA isolation kit (Ambion). Library preparation was done using QsRNA-seq protocol that mainly captures secondary siRNAs.
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| Contributor(s) |
Berkman MC, Hemo SB, Fishman A, Ben-Asher HW, T Lamm A, Henis-Korenblit S |
| Citation(s) |
32213289 |
| |
| Submission date |
Mar 27, 2019 |
| Last update date |
Apr 03, 2020 |
| Contact name |
sivan Korenblit |
| E-mail(s) |
sivankorenblit@gmail.com
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| Organization name |
Bar-Ilan University
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| Department |
Faculty of Life Sciences
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| Street address |
Life Sciences Building (212) , mailbox 63, Bar-Ian University
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| City |
Ramat Gan |
| State/province |
- None - |
| ZIP/Postal code |
5290002 |
| Country |
Israel |
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| Platforms (1) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA529404 |
| SRA |
SRP189600 |
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