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| Status |
Public on Jan 17, 2020 |
| Title |
C. elegans establishes germline versus soma by balancing inherited histone methylation [ChIP-seq] |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
In C. elegans, the H3K4me2 demethylase, SPR-5, and the H3K9 methyltransferase, MET-2, are maternally deposited into the oocyte where they reprogram histone methylation to prevent somatic expression of germline genes. Here, we show that the progeny of spr-5; met-2 mutants display a severe developmental delay that is associated with the ectopic expression of germline genes targeted by the H3K36me2/3 methyltransferase, MES-4. Maternally deposited MES-4 maintains H3K36me2/3 at a subset of germline genes (hereafter referred to as MES-4 germline genes) in a transcription-independent manner, and this is required for germline proliferation in the subsequent generation. By performing ChIP-seq on L1 progeny from spr-5; met-2 mutants, we find that MES-4 germline genes ectopically accumulate H3K36me3 in somatic tissues. Additionally, knocking down MES-4 suppresses the ectopic expression of MES-4 germline genes and rescues the developmental delay. These data suggest a model where SPR-5, MET-2 and MES-4 carefully balance the inheritance of histone methylation from the parental germline to ensure the proper specification of germline versus soma in the progeny. Without SPR-5; MET-2 maternal reprogramming, somatic cells struggle to specify their proper cell fate amongst the background noise of inappropriate germline gene transcription, leading to a severe developmental delay.
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| Overall design |
H3K36me3 ChIP-seq analysis on wild-type (N2) and spr-5; met-2 L1 progeny.
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| Web link |
https://doi.org/10.1242/dev.196600
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| |
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| Contributor(s) |
Carpenter BS, Lee TW, Plott CF, Brockett JS, Myrick DA, Katz DJ |
| Citation missing |
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| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| F32 GM126734 |
Complex interactions regulate histone methylation reprogramming at fertilization |
EMORY UNIVERSITY |
Brandon Scott Carpenter |
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| |
| Submission date |
Jan 16, 2020 |
| Last update date |
Jan 18, 2021 |
| Contact name |
Brandon S. Carpenter |
| E-mail(s) |
brandon.scott.carpenter@emory.edu
|
| Organization name |
Emory University
|
| Department |
Cell Biology
|
| Lab |
435
|
| Street address |
615 Michael St., SOM: Cell Biology Dept.
|
| City |
Atlanta |
| State/province |
Georgia |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE143839 |
C. elegans establishes germline versus soma by balancing inherited histone methylation |
|
| Relations |
| BioProject |
PRJNA601803 |
| SRA |
SRP242470 |
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