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| Status |
Public on May 04, 2020 |
| Title |
microRNA form extracellular vesicles-enriched preparations of the free-livingnematode Caenorhabditis elegans (strain N2, Bristol) cultured in vitro |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Total RNA secreted or excreted by C. elegans incubated for 5h or 24h in M9 culture media and isolated. Extracellular vesicles were isolated by differential ultracentrifugation, and subjected to miRNA sequencing.
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| Overall design |
Synchronized young adults (hermaphrodites) were incubated in liquid culture media for either 5 h or 24 h
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| Web link |
https://www.frontiersin.org/articles/10.3389/fcimb.2020.00217/full#supplementary-material
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| |
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| Contributor(s) |
Tritten L, Soichot J, Duguet T |
| Citation(s) |
32523895 |
| |
| Submission date |
Jan 27, 2020 |
| Last update date |
Jun 17, 2020 |
| Contact name |
Lucienne Tritten |
| E-mail(s) |
lucienne.tritten@mcgill.ca
|
| Organization name |
McGill University
|
| Department |
Institute of Parasitology
|
| Street address |
21111 Lakeshore Road
|
| City |
Sainte-Anne-de-Bellevue |
| State/province |
QC |
| ZIP/Postal code |
H9X3V9 |
| Country |
Canada |
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| Platforms (1) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA603303 |
| SRA |
SRP245365 |