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| Status |
Public on May 27, 2020 |
| Title |
Caenorhabditis elegans AF4/FMR2 family homolog affl-2 regulates heat shock induced gene expression |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To mitigate the deleterious effects of temperature increases on cellular organization and proteotoxicity, organisms have developed mechanisms to respond to heat stress. In eukaryotes, HSF1 is the master regulator of the heat shock transcriptional response, but the heat shock response pathway is not yet fully understood. From a forward genetic screen for suppressors of heat shock induced gene expression in Caenorhabditis elegans, we found a new allele of hsf-1 that alters its DNA-binding domain, and we found three additional alleles of sup-45, a previously molecularly uncharacterized genetic locus. We identified sup-45 as one of the two hitherto unknown C. elegans orthologs of the human AF4/FMR2 family proteins, which are involved in regulation of transcriptional elongation rate. We thus renamed sup-45 as affl-2 (AF4/FMR2-Like). Through RNA-seq, we demonstrated that affl-2 mutants are deficient in heat shock induced transcription. Additionally, affl-2 mutants have herniated intestines, while worms lacking its sole paralog (affl-1) appear wild type. AFFL-2 is a broadly expressed nuclear protein, and nuclear localization of AFFL-2 is necessary for its role in heat shock response. affl-2 and its paralog are not essential for proper HSF-1 expression and localization after heat shock, which suggests that affl-2 may function downstream or parallel of hsf-1. Our characterization of affl-2 provides insights into the regulation of heat shock induced gene expression to protect against heat stress.
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| Overall design |
We measured the effect of the affl-2(sy975) mutation on heat shock induced transcriptional response.
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| Web link |
https://www.genetics.org/content/215/4/1039
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| Contributor(s) |
Walton SJ, Wang H, Quintero-Cadena P, Bateman A, Sternberg PW |
| Citation(s) |
32518061 |
| |
| Submission date |
May 26, 2020 |
| Last update date |
Aug 27, 2020 |
| Contact name |
Sophie Jean Walton |
| E-mail(s) |
sophie.walton.98@gmail.com
|
| Phone |
6505220488
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| Organization name |
California Institute of Technology
|
| Department |
Biology and Biological Engineering
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| Lab |
Sternberg
|
| Street address |
346 Lakeview Way
|
| City |
Emerald Hills |
| State/province |
CA |
| ZIP/Postal code |
94062 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA635013 |
| SRA |
SRP264727 |
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