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| Status |
Public on Sep 30, 2020 |
| Title |
RNA-Sequencing used to identify the targets of NHR-49, a transcription factor that acts in distinct tissues to promote longevity versus immunity |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Previously, we identified NHR-49, functional homolog of the key vertebrate lipid metabolism regulator HNF4/PPARa, as essential for the longevity of Germiline Strem Cell (GSC)-less animals. Since longevity and stress resistance have been reported to be strongly linked, we examined the role of NHR-49 in resistance against the human opportunistic pathogen Pseudomonas aeruginosa, strain PA14. As a part of this effort we employed RNA-Sequencing to determine the downstream targets of NHR-49 in GSC-less animals.
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| |
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| Overall design |
Comparison of adult day 2 whole worm NGS-derived transcriptomic profiles of nhr-49;glp-1 and glp-1 animals
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| Contributor(s) |
Amrit FR, Ghazi A |
| Citation(s) |
34156142 |
| |
| Submission date |
Sep 29, 2020 |
| Last update date |
Jul 20, 2021 |
| Contact name |
Francis Amrit Raj Gandhi |
| Organization name |
University of Pittsburgh
|
| Street address |
4401 Penn Ave
|
| City |
Pittsburgh |
| ZIP/Postal code |
15224 |
| Country |
USA |
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| Platforms (1) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA666358 |
| SRA |
SRP285776 |
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