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| Status |
Public on May 26, 2022 |
| Title |
C. elegans small RNA-seq profiling of three different lotr-1 mutant alleles |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Proteins containing LOTUS and Tudor domains have critical roles in the germline, including in small RNA biology. LOTR-1 is the only protein expressed in the germline of C. elegans which has LOTUS and Tudor domains. Here, to understand the influence of LOTR-1 in small RNA biology, we sequenced small RNAs of wild-type and lotr-1 mutant animals.
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| Overall design |
Small RNA-seq profiling of wild-type and lotr-1 mutant worms. The three mutants analysed in this experiment have different deletions in the lotr-1 gene (details are described in the associated paper). RNA was treated with RppH prior to library preparation.
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| Contributor(s) |
Almeida MV, Karaulanov E, Ketting RF |
| Citation(s) |
35657999 |
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| Submission date |
Apr 14, 2021 |
| Last update date |
Jun 07, 2022 |
| Contact name |
Emil Karaulanov |
| Organization name |
Institute of Molecular Biology
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| Lab |
Bioinformatics Core Facility
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| Street address |
Ackermannweg 4
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| City |
Mainz |
| ZIP/Postal code |
55128 |
| Country |
Germany |
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| Platforms (1) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE192794 |
LOTR-1 interacts with ZNFX-1 to balance epigenetic signals in the C. elegans germline |
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| Relations |
| BioProject |
PRJNA721953 |
| SRA |
SRP314829 |
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