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| Status |
Public on Dec 31, 2022 |
| Title |
Whole-organism C. elegans L2 GRH-1 ChIP-seq (experiment 2 of 2) |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We performed ChIP-seq to determine chromatin regions binding Transcription Factor GRH-1, and thereby potential binding motifs and sites, in C. elegans (larval stage 2) whole organisms.
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| Overall design |
One sample was used as "input", ie non-specific pulldown. 1 new GRH-1-specific pulldown sample was generated. Samples were collected at two time points to broadly cover the L2 stage, before pooling. Additionally, two samples (1 input and 1 pulldown, 500mM salt) from experiment 1 were re-extracted, prepared and sequenced.
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| Contributor(s) |
Azzi C, Braun K, Smith AA, Gaidatzis D, Meeuse MW, Grosshans H |
| Citation(s) |
36688410 |
| |
| Submission date |
Sep 16, 2022 |
| Last update date |
Apr 03, 2023 |
| Contact name |
Helge Grosshans |
| E-mail(s) |
helge.grosshans@fmi.ch
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| Organization name |
Friedrich Miescher Institute for Biomedical Research
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| Department |
Multicellular Systems
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| Lab |
Biological clocks and timers in development
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| Street address |
Maulbeerstrasse 66
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| City |
Basel |
| State/province |
Basel-Stadt |
| ZIP/Postal code |
4058 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE213510 |
Whole-organism C. elegans L2 GRH-1 ChIP-seq |
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| Relations |
| BioProject |
PRJNA881279 |
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