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| Status |
Public on Feb 10, 2025 |
| Title |
Impact of human anti-O-specific polysaccharide (OSP) antibody on Vibrio cholerae O1 in complex media containing mucin |
| Organism |
Vibrio cholerae C6706 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Antibodies targeting the O-specific polysaccharide (OSP) of Vibrio cholerae O1 are a main determinant of protection against cholera. These antibodies can agglutinate bacteria, and can also directly inhibit V. cholerae motility, including at sub-agglutinating conditions. In order to evaluate for possible additional impacts of OSP-specific antibody on V. cholerae, we assessed the transcriptional profile of V. cholerae exposed to an anti-OSP human monoclonal antibody (G1), including in the presence of mucin, the principal component of intestinal mucous. We identified a subset of genes whose expression was significantly altered in the presence of anti-OSP antibody and mucin, including those involved in V. cholerae metabolism, transport, stress response, biofilm formation, motility, and secondary messenger signaling. Our results suggest a broad impact of anti-OSP antibodies on V. cholerae in the presence of mucin and identify several possible mechanisms by which anti-OSP antibodies might protect against cholera.
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| Overall design |
Vibrio cholerae C6706 bacteria cultured under toxin-inducing conditions were diluted to a subagglutinating optical density (600 nm) of ≤0.1 and exposed to two concentrations (0.0125 µM and 0.125 µM) of human monoclonal immunoglobulin (Ig) G1 targeting V. cholerae O-specific polysaccharide component of LPS (clone G1 - CF21.2.G01) or anti-flagellin antibody as a control (clone B12 -AT11.1.B12) in either Luria Bertani (LB) broth or LB containing 1% w/v porcine stomach mucin (LBM).
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| Contributor(s) |
Ryan ET, Verma S |
| Citation missing |
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| Submission date |
Jan 24, 2025 |
| Last update date |
Feb 10, 2025 |
| Contact name |
Smriti Verma |
| E-mail(s) |
SVERMA5@mgh.harvard.edu
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| Organization name |
Massachusetts General Hospital
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| Department |
Department of Infectious Diseases
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| Lab |
Edward T Ryan Lab
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| Street address |
55 Fruit Street
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
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| Platforms (1) |
| GPL35328 |
NextSeq 2000 (Vibrio cholerae C6706) |
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| Samples (20)
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| GSM8756956 |
1h/LB_G1_x-rep2, SVb3-6 |
| GSM8756957 |
1h/LB_G1_xxx-rep1, SVb3-11 |
| GSM8756958 |
1h/LB_G1_xxx-rep2, SVb3-12 |
| GSM8756959 |
1h/LB_B12_x-rep1, SVb3-14 |
| GSM8756960 |
1h/LB_B12_x-rep2, SVb3-15 |
| GSM8756961 |
1h/LB_B12_xxx-rep1, SVb3-20 |
| GSM8756962 |
1h/LB_B12_xxx-rep2, SVb3-21 |
| GSM8756963 |
1h/LBM-rep1, SVb3-31 |
| GSM8756964 |
1h/LBM-rep2, SVb3-33 |
| GSM8756965 |
1h/LBM_G1_x-rep1, SVb3-34 |
| GSM8756966 |
1h/LBM_G1_x-rep2, SVb3-36 |
| GSM8756967 |
1h/LBM_G1_xxx-rep1, SVb3-40 |
| GSM8756968 |
1h/LBM_G1_xxx-rep2, SVb3-42 |
| GSM8756969 |
1h/LBM_B12_x_rep1, SVb3-43 |
| GSM8756970 |
1h/LBM_B12_x-rep2, SVb3-45 |
| GSM8756971 |
1h/LBM_B12_xxx-rep1, SVb3-50 |
| GSM8756972 |
1h/LBM_B12_xxx-rep2, SVb3-51 |
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| Relations |
| BioProject |
PRJNA1215427 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE287993_RAW.tar |
1.5 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
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