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| Status |
Public on Nov 13, 2025 |
| Title |
Single cell resolution of an open chromatin signature in persister tumor cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
To characterize transcriptional and chromatin accessibility changes that promote survival of residual cancer following chemotherapy, we performed single-cell multiomic profiling (snRNA+snATAC) on a cohort of non-malignant fallopian tube, treatment-naive, and neoadjuvant chemotherapy (NACT)-treated high-grade serous ovarian cancer (HGSOC) samples. From this analysis, we identified an epigenetic signature that precedes expression and defines the drug-tolerant persister state.
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| Overall design |
Nuclei from ovarian cancer tissue were isolated and processed for single cell Multiome sequencing following 10x Genomics protocol.
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| Contributor(s) |
Dumbrava M, Mohammed Ismail W, Sandoval L, Mazzone A, Hou X, Harrington S, Kauffmann SH, Weroha SJ, Gaspar-Maia A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 01, 2025 |
| Last update date |
Nov 14, 2025 |
| Contact name |
Alexandre Gaspar-Maia |
| Organization name |
Mayo Clinic
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| Street address |
200 First street SW
|
| City |
Rochester |
| State/province |
MN |
| ZIP/Postal code |
55905 |
| Country |
USA |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA1244735 |
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