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| Status |
Public on Dec 14, 2025 |
| Title |
NGLY1-dependent conversion of N-glycosylated N to D is essential for transcription of proteasome genes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the sequence editing-specific effects on Nrf1 functions, we utilized glycan-less Nrf1 mutants, 9NA and 9ND, in which all nine of the putative N-glycosylation sites (N-X-S/T; asparagine (N) serine (S), threonine (T), and X is any amino acid except proline) were replaced with alanine (A) and aspartic acid (D), respectively. To comprehensively understand the effects of Nrf1 sequence editing on the transcriptome, microarray analysis was performed on NGLY1-KO HeLa cells stably expressing 9NA and 9ND.
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| Overall design |
Three independent total RNA samples from 20 nM Btz (for 15 h) treated NGLY1-KO HeLa cells without/ with stably expressing 9NA-Nrf1-HA or 9ND-Nrf1-HA were purified.
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| Contributor(s) |
Nishito Y, Yoshida Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 03, 2025 |
| Last update date |
Dec 17, 2025 |
| Contact name |
Yukiko Yoshida |
| E-mail(s) |
yoshida-yk@igakuken.or.jp
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| Organization name |
Tokyo Motrolopotan Institute of Medical Science
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| Department |
Department of Basic Medical Sciences
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| Lab |
Laboratory of Protein Metabolism
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| Street address |
2-1-6, Kamikitazawa, Setagaya-ku
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| City |
Tokyo |
| ZIP/Postal code |
156-8506 |
| Country |
Japan |
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| Platforms (1) |
| GPL10332 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Feature Number version) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA1285842 |
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