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| Status |
Public on Dec 14, 2025 |
| Title |
Inflammation-induced lncRNA SNHG1 Orchestrates Spermatogonium Development in Non-obstructive Azoospermia via IL-17A Signaling Pathway |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Non-obstructive azoospermia (NOA) is a critical subtype of male infertility associated with inflammation. However, the molecular mechanisms underlying this phenomenon remain poorly understood. This study investigated the role of the inflammation-activated long non-coding RNA SNHG1 in NOA pathogenesis. Using lipopolysaccharide (LPS)-induced orchitis mouse models and spermatogonium cell lines (GC-1 spg and TCAM-2), we observed that both SNHG1 and the transcription factor SP1 were significantly upregulated, correlating with spermatogonium proliferation and loss of stemness. Mechanistically, SP1 directly binds to and transcriptionally activates the SNHG1 promoter, whereas SNHG1 knockdown rescued LPS-induced spermatogonium dysfunction without affecting SP1 expression. RNA-seq revealed that SNHG1 overexpression activated the IL-17A signaling pathway. Notably, IL-17A receptor blockade (Brodalumab) reversed the SNHG1-mediated proliferation arrest and stemness. Our findings demonstrated that the SP1-SNHG1-IL-17A axis drives inflammatory spermatogenic failure, suggesting that IL-17A inhibition is a potential therapeutic strategy for NOA.
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| Overall design |
RNA-seq profiling of Vector-control and SNHG1-overexpressing GC-1 spg cells at 24 hours post-transfection.
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| Contributor(s) |
Zhu Y, Li M, Zhan X, Liu L, Chen C, Zhou Y, He P, Hua R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 10, 2025 |
| Last update date |
Dec 14, 2025 |
| Contact name |
Rui Hua |
| E-mail(s) |
ruih218@smu.edu.cn
|
| Phone |
13889902677
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| Organization name |
Nanfang Hospital, Southern Medical University
|
| Department |
Department of Obstetrics and Gynecology
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| Street address |
838 Guangzhou North Avenue
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| City |
Guangzhou |
| State/province |
GuangDong |
| ZIP/Postal code |
510515 |
| Country |
China |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| GSM9362469 |
GC-1 spg cells, Ov_SNHG1_1_QN202402240001-23 |
| GSM9362470 |
GC-1 spg cells, Ov_SNHG1_2_QN202402240001-23 |
| GSM9362471 |
GC-1 spg cells, Ov_SNHG1_3_QN202402240001-23 |
| GSM9362472 |
GC-1 spg cells, Vector_1_QN202402240001-23 |
| GSM9362473 |
GC-1 spg cells, Vector_2_QN202402240001-23 |
| GSM9362474 |
GC-1 spg cells, Vector_3_QN202402240001-23 |
|
| Relations |
| BioProject |
PRJNA1378695 |
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