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| Status |
Public on Dec 14, 2025 |
| Title |
scRNA-seq data of hepatic immune cells isolated from Alb-WT Bicd2fl/fl and Alb-Cre Bicd2fl/fl mice subjected to S-100 induced AIH model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To determine the changes in liver immune cells upon hepatic Bicd2 deficiency, we performed scRNA-seq to analyze the hepatic immune microenvironment in Alb-WT Bicd2fl/fl and Alb-Cre Bicd2fl/fl mice subjected to S-100 induced AIH model.
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| Overall design |
Alb-WT Bicd2fl/fl and Alb-Cre Bicd2fl/fl mice subjected to S-100 induced AIH model. To obtain hepatic immune cells, liver tissues were minced and grinded in pre-cold FACS buffer (PBS containing 1% FBS), followed by filtration through a 70 μm cell strainer. Lymphocytes were isolated through 40/70% Percoll (Cytiva, Cat# 17089109) by gradient centrifuging 800 × g for 20 min. Cells located at the inter-layer were collected. After removing red blood cells resuspended with ACK lysis buffer, living cells were sorted and pooled using the 10× Genomics platform and sequenced on an Illumina NextSeq sequencer.
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| Contributor(s) |
Tian W, Wang W, Lv D |
| Citation missing |
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| Submission date |
Dec 10, 2025 |
| Last update date |
Dec 15, 2025 |
| Contact name |
Weixuan Wang |
| E-mail(s) |
2110305109@stu.pku.edu.cn
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| Organization name |
Peking University
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| Street address |
No. 38 Xueyuan Road, Haidian District
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| City |
Beijing |
| ZIP/Postal code |
100191 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA1378844 |
