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| Status |
Public on Oct 01, 2015 |
| Title |
Expression data from Ezh2 conditional C2 iMEFs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Polycomb Repressive Complex 2 (PRC2) plays a key role in controlling transcriptional repression. It is thought to act at the level of the chromatin, where its enzymatic subunits Ezh1 and Ezh2 catalyse the di/tri-methylation of histone H3 on its lysine 27 (H3K27me3).
We sought to assess several parameters of PRC2-mediated transcriptional repression. To this end we performed a comparative analysis of mES and iMEF cells following deletion of Ezh2, a time-course analysis following deletion of Ezh2 in iMEFs as well as rescue experiments with Ezh1 and Ezh2 before or after deletion of Ezh2.
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| Overall design |
In a first experiment, we performed a transcriptome analysis of mES and iMEF cells either Ezh2 WT or Ezh2-mutant. In a second experiment, we performed a time-course analysis of a clone of Ezh2-conditional iMEFs (clone C2), 0 (control), 5, 18 and 32 days after addition of Tamoxifen. In a third experiment, we analysed the transcriptome of C2 cells either i) wild-type for Ezh2, ii) in the presence of overexpressed exogenous Ezh1 or Ezh2, iii) Ezh2-mutant, and iv) with Ezh1/Ezh2 rescue either pre- or post-deletion of endogenous Ezh2.
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| Contributor(s) |
Teissandier A, Zeitouni B, Margueron R |
| Citation(s) |
26637281 |
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| Submission date |
Jul 11, 2014 |
| Last update date |
Apr 18, 2017 |
| Contact name |
Nicolas Servant |
| E-mail(s) |
Nicolas.Servant@curie.fr
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| Organization name |
Institut Curie
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| Street address |
26 rue d'ulm
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| City |
Paris Cedex 05 |
| ZIP/Postal code |
75248 |
| Country |
France |
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| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (18)
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| GSM1435462 |
experiment 2: vehicle-treated replicate 1 |
| GSM1435463 |
experiment 2: vehicle-treated replicate 2 |
| GSM1435464 |
experiment 2: TAM-treated day 5 replicate 1 |
| GSM1435465 |
experiment 2: TAM-treated day 5 replicate 2 |
| GSM1435466 |
experiment 2: TAM-treated day 18 replicate 1 |
| GSM1435467 |
experiment 2: TAM-treated day 18 replicate 2 |
| GSM1435468 |
experiment 2: TAM-treated day 32 replicate 1 |
| GSM1435469 |
experiment 2: TAM-treated day 32 replicate 2 |
| GSM1435470 |
experiment 3: control replicate 1 |
| GSM1435471 |
experiment 3: control replicate 2 |
| GSM1435474 |
experiment 3: Ezh2 -/-, replicate 1 |
| GSM1435475 |
experiment 3: Ezh2 -/-, replicate 2 |
| GSM1435476 |
experiment 3: Ezh1 pre-rescue condition, replicate 1 |
| GSM1435477 |
experiment 3: Ezh1 pre-rescue condition, replicate 2 |
| GSM1435478 |
experiment 3: Ezh2 pre-rescue condition, replicate 1 |
| GSM1435479 |
experiment 3: Ezh2 pre-rescue condition, replicate 2 |
| GSM1435480 |
experiment 3: Ezh2 post-rescue condition, replicate 1 |
| GSM1435481 |
experiment 3: Ezh2 post-rescue condition, replicate 2 |
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| This SubSeries is part of SuperSeries: |
| GSE59427 |
Impaired PRC2 activity promotes transcriptional instability and favors breast tumorigenesis |
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| Relations |
| BioProject |
PRJNA255241 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE59346_RAW.tar |
79.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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