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| Status |
Public on Dec 04, 2017 |
| Title |
Deep sequencing of poly(A)-selected RNAs from whole nematode C. elegans during aging |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
MicroRNAs (miRNAs) have been implicated in gene regulation in many complex processes, including aging. Moreover, expression levels of many miRNAs are altered during aging, though we know little about the mechanisms that regulate this process. To address these issues, we investigated the network of regulatory factors that interact with miRNAs during aging. We found miR-71, a known promoter of normal lifespan, to be an especially important regulator of alg-1/Argonaute. Here we show that increased ALG-1 expression in aging mir-71 loss-of-function mutants was associated with an increased global miRNA abundance and deregulation of mRNA expression. Further, variability in gene expression and lifespan are diminished in mir-71 mutants. Thus, miR-71 appears to promote normal lifespan by directly repressing the expression of alg-1 and indirectly regulating miRNA expression levels and noise. Our findings demonstrate the broad impacts of miRNAs on gene regulation during aging.
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| Overall design |
RNA profiles (poly(A)-selected RNA) from Day 0 adulthood and Day 5 adulthood C. elegans nematodes. 12 samples total, in biological triplicate at each time point from wildtype control (N2) and mir-71(n4115) strains. Generated using Illumina HiSeq 2500.
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| Contributor(s) |
Inukai S, Slack FJ |
| Citation(s) |
29114017 |
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| Submission date |
Aug 20, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Sachi Inukai |
| E-mail(s) |
sachi.inukai@yale.edu
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| Organization name |
Yale University
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| Street address |
219 Prospect St
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| City |
New Haven |
| State/province |
Connecticut |
| ZIP/Postal code |
06511 |
| Country |
USA |
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| Platforms (1) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
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| Samples (12)
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| GSM1858284 |
N2 Day 0, replicate 1 [poly(A) RNA] |
| GSM1858285 |
N2 Day 5, replicate 1 [poly(A) RNA] |
| GSM1858286 |
mir-71(n4115) Day 0, replicate 1 [poly(A) RNA] |
| GSM1858287 |
mir-71(n4115) Day 5, replicate 1 [poly(A) RNA] |
| GSM1858288 |
N2 Day 0, replicate 2 [poly(A) RNA] |
| GSM1858289 |
N2 Day 5, replicate 2 [poly(A) RNA] |
| GSM1858290 |
mir-71(n4115) Day 0, replicate 2 [poly(A) RNA] |
| GSM1858291 |
mir-71(n4115) Day 5, replicate 2 [poly(A) RNA] |
| GSM1858292 |
N2 Day 0, replicate 3 [poly(A) RNA] |
| GSM1858293 |
N2 Day 5, replicate 3 [poly(A) RNA] |
| GSM1858294 |
mir-71(n4115) Day 0, replicate 3 [poly(A) RNA] |
| GSM1858295 |
mir-71(n4115) Day 5, replicate 3 [poly(A) RNA] |
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| This SubSeries is part of SuperSeries: |
| GSE72234 |
RNA-seq and small RNA-seq analysis of N2 wildtype and mir-71(n4115) C. elegans during aging |
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| Relations |
| BioProject |
PRJNA293449 |
| SRA |
SRP062670 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE72232_RNA-seq_combined_counts.txt.gz |
589.7 Kb |
(ftp)(http) |
TXT |
| GSE72232_RNA-seq_combined_counts_normalized_rlog.txt.gz |
1.8 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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