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Series GSE75892 Query DataSets for GSE75892
Status Public on Dec 08, 2018
Title Nuclear phosphorylated Dicer processes double-stranded RNA in response to DNA damage
Organism Homo sapiens
Experiment type Other
Genome binding/occupancy profiling by high throughput sequencing
Summary The endonuclease Dicer is a key component of the human RNA interference (RNAi) pathway and known for its role in cytoplasmic micro RNA (miRNA) production. Recent findings suggest that non-canonical Dicer generates small non-coding RNA (ncRNA) to mediate the DNA damage response (DDR). Here we show that human Dicer is phosphorylated in the platform-PAZ-connector helix cassette (S1016) upon induction of DNA damage. Phosphorylated Dicer (p-Dicer) accumulates in the nucleus and is recruited to DNA double-strand breaks (DSBs). We further demonstrate that turnover of damage-induced nuclear dsRNA requires additional phosphorylation of carboxy-terminal Dicer residues (S1728 and S1852). DNA damage-induced nuclear Dicer accumulation is conserved in mammals. Dicer depletion causes spontaneous DNA damage and delays DNA repair. Taken together, we place Dicer in context of the DDR by demonstrating DNA damage-inducible phospho-switch that causes localised processing of nuclear dsRNA by p-Dicer to promote DNA repair.
 
Overall design We have analysed ChIP samples from damaged and non-damaged cells, using an anti-Dicer antibody. Non-damaged sample serves as physiological control.
 
Contributor(s) Burger K, Schlackow M, Hester SS, Mohammed S, Gullerova M
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Submission date Dec 10, 2015
Last update date May 15, 2019
Contact name Monika Gullerova
E-mail(s) monika.gullerova@path.ox.ac.uk
Phone +44794220892
Organization name University of Oxford
Department Sir William Dunn School of Pathology
Street address South Parks Road
City Oxford
ZIP/Postal code OX1 3RE
Country United Kingdom
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (2)
GSM2532748 Dicer DNA Damage
GSM2532749 Dicer no DNA Damage
Relations
BioProject PRJNA305622
SRA SRP067228

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Supplementary file Size Download File type/resource
GSE75892_RAW.tar 147.3 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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