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Series GSE76980 Query DataSets for GSE76980
Status Public on Jan 19, 2016
Title iPSC RNA-seq upon CRISPRn or CRISPRi manipulation
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Assess the on- and off-target effects of dox-inducible CRISPR/Cas9 and CRISPRi constructs in a human iPS cell line.
 
Overall design Transcript quantification of 3 cell lines, each plus or minus doxycycline and with or without specific single guide RNAs (sgRNAs), with 2 biological replicates each.
 
Contributor(s) Mandegar MA, Huebsch N, Balandina E, Shin E, Truong A, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, Weissman JS, Qi LS, So P, Conklin BR
Citation(s) 26971820
BioProject PRJNA307261
Submission date Jan 19, 2016
Last update date May 15, 2019
Contact name Max A Horlbeck
Organization name UCSF
Department Cellular and Molecular Pharmacology
Lab Weissman Lab
Street address 1700 4th Street
City San Francsico
State/province CA
ZIP/Postal code 94158
Country USA
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (16)
GSM2041869 WTC, -dox, RepA
GSM2041870 WTC, +dox, RepA
GSM2041871 CRISPRn, -dox, RepA
Relations
SRA SRP067928

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE76980_iPSC_combined_read_counts.tsv.gz 3.8 Mb (ftp)(http) TSV
SRA Run SelectorHelp
Processed data are available on Series record
Raw data are available in SRA

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