 |
 |
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Jan 19, 2016 |
Title |
iPSC RNA-seq upon CRISPRn or CRISPRi manipulation |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
Assess the on- and off-target effects of dox-inducible CRISPR/Cas9 and CRISPRi constructs in a human iPS cell line.
|
|
|
Overall design |
Transcript quantification of 3 cell lines, each plus or minus doxycycline and with or without specific single guide RNAs (sgRNAs), with 2 biological replicates each.
|
|
|
Contributor(s) |
Mandegar MA, Huebsch N, Balandina E, Shin E, Truong A, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, Weissman JS, Qi LS, So P, Conklin BR |
Citation(s) |
26971820 |
BioProject |
PRJNA307261 |
|
Submission date |
Jan 19, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Max A Horlbeck |
Organization name |
UCSF
|
Department |
Cellular and Molecular Pharmacology
|
Lab |
Weissman Lab
|
Street address |
1700 4th Street
|
City |
San Francsico |
State/province |
CA |
ZIP/Postal code |
94158 |
Country |
USA |
|
|
Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
Samples (16)
|
GSM2041872 |
CRISPRn, +dox, RepA |
GSM2041873 |
CRISPRi, -dox, RepA |
GSM2041874 |
CRISPRi, +dox, RepA |
GSM2041875 |
CRISPRi, sgGCaMP, -dox, RepA |
GSM2041876 |
CRISPRi, sgGcaMP, +dox, RepA |
GSM2041877 |
WTC, -dox, RepB |
GSM2041878 |
WTC, +dox, RepB |
GSM2041879 |
CRISPRn, -dox, RepB |
GSM2041880 |
CRISPRn, +dox, RepB |
GSM2041881 |
CRISPRi, -dox, RepB |
GSM2041882 |
CRISPRi, +dox, RepB |
GSM2041883 |
CRISPRi, sgGCaMP, -dox, RepB |
GSM2041884 |
CRISPRi, sgGcaMP, +dox, RepB |
|
Relations |
SRA |
SRP067928 |
Supplementary file |
Size |
Download |
File type/resource |
GSE76980_iPSC_combined_read_counts.tsv.gz |
3.8 Mb |
(ftp)(http) |
TSV |
SRA Run Selector |
Processed data are available on Series record |
Raw data are available in SRA |
|
|
|
|
 |