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| Status |
Public on May 24, 2016 |
| Title |
Effects of blocking IL-17A on lung gene expression after ozone exposure in obese (db/db) mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Obese mice have an augmented response to Ozone exposure (2 ppm for 3 hours and studied 24 hours later), including increase airway hyperresponsiveness and BAL inflammation when compared to WT ozone exposed mice. When IL-17A is block with systemic injected of IL-17A antibodies there is a decrease of response in the obese mice. To determine the mechanism of action, RNA was extracted from the lungs of IL-17A and Isotype injected ozone exposed obese mice and microarray analysis of gene expression was performed.
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| Overall design |
Lungs were harvested from db/db mice exposed to air and ozone and injected with anti-IL-17A and isotype.
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| Contributor(s) |
Shore SA, Mathews JA, Kasahara DI |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 23, 2016 |
| Last update date |
Apr 18, 2017 |
| Contact name |
Stephanie A Shore |
| E-mail(s) |
sshore@hsph.harvard.edu
|
| Organization name |
Harvard School of Public Health
|
| Department |
Environmental Health
|
| Lab |
Shore Lab
|
| Street address |
665 Huntigton Av Bld1 room 307
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA322558 |
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