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Series GSE82045 Query DataSets for GSE82045
Status Public on Mar 12, 2018
Title Global delay in nascent strand DNA methylation
Organism Homo sapiens
Experiment type Methylation profiling by high throughput sequencing
Summary BrdU labeling combined with genome-wide bisulfite sequencing (Repli-BS-Seq) reveals a global lag in the inheritance of CpG methylation following DNA replication, giving rise to cell-cycle-dependent epigenetic variation.
 
Overall design In this study, we pulsed cells with BrdU to label nascent DNA and then performed whole genome bisulfite sequencing on either the immunoprecipitated nascent DNA or bulk DNA.
 
Contributor(s) Charlton J, Downing TL, Smith ZD, Gu H, Clement K, Pop R, Akopian V, Klages S, Tasnkov AM, Santos D, Timmermann B, Ziller MJ, Kiskinis E, Gnirke A, Meissner A
Citation(s) 29531288
Submission date May 31, 2016
Last update date Jul 25, 2021
Contact name Jocelyn Charlton
E-mail(s) jocelyn_charlton@harvard.edu
Organization name Harvard University
Department Stem Cell and Regenerative Biology
Lab Meissner Lab
Street address 7 Divinity Avenue
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (28)
GSM2182515 HUES64_WT_S1fraction_1hBrdu_0Chase_nascent
GSM2182516 HUES64_WT_S2fraction_1hBrdu_0Chase_nascent
GSM2182517 HUES64_WT_S3fraction_1hBrdu_0Chase_nascent
Relations
BioProject PRJNA323802
SRA SRP075910

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE82045_RAW.tar 2.8 Gb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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