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Series GSE84868 Query DataSets for GSE84868
Status Public on Feb 01, 2017
Title Expression data from control and WHSC1 siRNA treated PC3 cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We conduct transcriptome comparison of control and WHSC1 depleted PC3 cells to gain genomic insights on the biological processes that WHSC1 is involved in prostate cancer cells. More than 2000 known genes were found changed in the WHSC1 depleted cells. Ingenuity Pathway Analysis (IPA) and Gene Set Enrichment Analysis (GSEA) show that the most prominent altered pathways in the WHSC1 depleted cells are related to regulation of actin-based motility, mTOR signaling pathway.
 
Overall design Genome-wide expression profiling of PC-3 cells transiently transduced with control siRNA in comparison to two independent siRNAs against WHSC1 and total RNA was isolated 72 hours later. Each is tested in duplicate.
 
Contributor(s) Qin J, Li N
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Submission date Jul 26, 2016
Last update date May 15, 2019
Contact name Ni Li
E-mail(s) lini@sibs.ac.cn
Organization name Institute of Health Sciences, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences
Lab Laboratory of Tumor Progression and Metastasis
Street address 320 Yueyang Road, Shanghai, 200025 P.R. China
City Shanghai
State/province Shanghai
ZIP/Postal code 200031
Country China
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (4)
GSM2252899 control RNASeq rep1
GSM2252900 control RNASeq rep2
GSM2252901 siWHSC1 RNASeq rep1
Relations
BioProject PRJNA335397
SRA SRP079918

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE84868_PC-3.control.and.WHSC1.knockdown.all.gene.FPKM.txt.gz 2.7 Mb (ftp)(http) TXT
GSE84868_RAW.tar 1.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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