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| Status |
Public on Oct 05, 2017 |
| Title |
H3K27Ac ChIP-seq for TL-om1 cells and primary ATL cell samples |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
ChIP-seq analysis was performed in adult T-cell leukemia/lymphoma cells to analyze acetylation of histone H3K27.
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| Overall design |
TL-Om1 and primary ATL cells were crosslinked with formaldehyde for 10 min. DNA was enriched by chromatin-immunoprecipitation (ChIP) and analyzed by Illumina Hiseq 4000. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against histone H3 lysine 27 acetylation (H3K27Ac: Abcam, ab4729).
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| |
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| Contributor(s) |
Wong RW, Ngoc PC, Leong WZ, Yam AW, Sanda T |
| Citation(s) |
28978570 |
| |
| Submission date |
Aug 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Phuong Cao Thi Ngoc |
| Organization name |
Lund University
|
| Street address |
BMC, B1222b, Klinikgatan 26
|
| City |
Lund |
| ZIP/Postal code |
SE-221 84 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (22)
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| This SubSeries is part of SuperSeries: |
| GSE85695 |
Identification of Super-enhancer-associated Cancer Genes Provides Novel Therapeutic Targets in Adult T-cell Leukemia/Lymphoma |
|
| Relations |
| BioProject |
PRJNA339128 |
| SRA |
SRP082212 |
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