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Status |
Public on Jul 15, 2020 |
Title |
The impact of YTHDF2 knockout on the distribution of 5-methylcytosine in RNA |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
5-methylcytosine is an important epigenetic modification found in both DNA and RNA. Transcriptome-wide mapping of 5-methylcytosine revealed that, after CRISPR-Cas9-mediated knockout of YTHDF2 gene, 545 sites displayed significant changes in 5-methylcytosine levels, with the majority of 5-methylcytosine sites in ribosomal RNA exhibiting substantially increased levels of methylation. Our data demonstrateda broader role of YTHDF2 in the post-transcriptional regulation of RNA.
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Overall design |
Combine RNA bisulfite conversion with next-generation sequencing to monitor the variation of 5-methylcytosine in the transcriptome of HEK293T and isogenic cells with YTHDF2 gene being knocked out.
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Contributor(s) |
Dai X, Li L, Li J, You C, Gonzalez G, Miao W, Hu J, Fu L, Xu Y, Gu W, Wang Y |
Citation missing |
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Submission date |
Aug 22, 2016 |
Last update date |
Jul 18, 2020 |
Contact name |
Weifeng Gu |
Organization name |
University of California at Riverside
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Department |
Molecular, Cell and Systems Biology
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Lab |
Weifeng Gu
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Street address |
900 University Ave
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City |
Riverside |
State/province |
California |
ZIP/Postal code |
92521 |
Country |
USA |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (6)
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Relations |
BioProject |
PRJNA339688 |
SRA |
SRP082495 |