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Series GSE89334 Query DataSets for GSE89334
Status Public on Jul 06, 2017
Title Discovery of Drug Candidates that Inhibit and Eliminate Zika Virus Infection in Fetal and Adult Brain
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Zika virus (ZIKV) infects fetal and adult human brains, and is associated with serious neurological complications including microcephaly and Guillain-Barré Syndrome (GBS). To date, no prophylactic or therapeutic treatment is available to prevent or treat ZIKV infection. Here, we performed a high content chemical screen using a library containing FDA-approved drugs or drug candidates. Two compounds, hippeastrine hydrobromide (HH) and amodiaquine dihydrochloride dihydrate (AQ), were discovered to inhibit ZIKV infection in human cortical neuron progenitor cells (hNPCs). HH was further validated to inhibit ZIKV infection and to rescue ZIKV-induced growth and differentiation defects in hNPCs and human fetal-like forebrain organoids. Finally, HH and AQ suppressed ZIKV infection in adult mouse brain in vivo. Strikingly, HH and AQ fully rescued the severe limb paralysis syndrome developed in ZIKV infected adult mice. This study identifies drug candidates for treatment of ZIKV infection and ZIKV-related neurological complications in fetal and adult patients.
 
Overall design 6 samples were included as following design: 1) neural progenitor cells (NPCs) with MOCK infection and DMSO treatment, 2) NPCs with MOCK infection and treated with AQ, 2) NPCs with MOCK infection and treated with HH, 4) NPCs infected with ZIKA (MR766 strain) and treated with DMSO, 5) NPCs infected with ZIKA and treated with AQ, 6) NPCs infected with ZIKA and treated with HH. All treatment above was lasted for 3 days.
 
Contributor(s) Zhou T, Tan L, Cederquist GY, Studer L, Chen S
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Submission date Oct 31, 2016
Last update date May 15, 2019
Contact name Shuibing Chen
E-mail(s) shuibing.chen@gmail.com
Phone 2127465431
Organization name Weill Cornell Medical College
Department Surgery
Street address A 827B, 1300 York Ave
City New York
State/province NY
ZIP/Postal code 10065
Country USA
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (6)
GSM2366234 NPC_M
GSM2366235 NPC_AQ
GSM2366236 NPC_HH
Relations
BioProject PRJNA351831
SRA SRP092330

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE89334_Processed_gene_expression.xls.gz 1.7 Mb (ftp)(http) XLS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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