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Status |
Public on Jul 26, 2017 |
Title |
The LncRNA NEAT1 Nests RNA Binding Proteins and the Microprocessor to Globally Enhance Pri-miRNA Processing |
Organism |
Homo sapiens |
Experiment type |
Other Non-coding RNA profiling by high throughput sequencing
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Summary |
MicroRNA biogenesis is known to be modulated by a variety of RNA binding proteins (RBPs), but in most cases, individual RBPs appear to influence the processing of a small number of selective targets. We herein report binding of the NONO/PSF heterodimer to hundreds of expressed pri-miRNAs in HeLa cells to globally enhance pri-miRNA processing by the Drosha/DGCR8 Microprocessor. As NONO/PSF are key components of paraspeckles organized by the lncRNA NEAT1, we find that NEAT1 also has profound effects on global pri-miRNA processing. Mechanistic dissection reveals that NEAT1 broadly interacts with NONO/PSF as well as many other RBPs, and that multiple RNA segments in NEAT1, including a “pseudo pri-miRNA” near its 3’ end, help attract the Microprocessor. These findings suggest a bird nest model for a large lncRNA to orchestrate efficient processing of an entire class of small RNAs in the nucleus.we used small RNA-seq to identify miRNA level in response to secific knockdowns relative to siGFP treatment control
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Overall design |
We profiled the miRNAs expression levels under conditions including siGFP(control), siNEAT1, siNEAT1_V2, siNONO, siPSF and siPSPC1 through small RNA-seq respectively. And we also examined the in vivo interactions between NONO/PSF and RNA through CLIP-seq.
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Contributor(s) |
Jiang L, Shao C, Zhou Y, Fu X |
Citation(s) |
28846091 |
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Submission date |
Nov 29, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Xiang-Dong Fu |
Organization name |
UC San Diego
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Department |
CMM
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Lab |
George Palade Laboratories
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Street address |
9500 Gilman Drive, Room 231
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City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92093-0651 |
Country |
USA |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (16)
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Relations |
BioProject |
PRJNA355281 |
SRA |
SRP094056 |