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Series GSE93911 Query DataSets for GSE93911
Status Public on Jan 22, 2018
Title Aberrant mRNA m6A methylation promotes the progression of endometrial cancer by altering Akt activity
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The N6-methyladenosine (m6A) methylation of mRNA is an emerging gene regulatory mechanism that controls the stability and translation of methylated transcripts in development and cell differentiation. Here, we show that aberrant m6A methylation plays key roles in the progression of endometrial cancer. About 70% of endometrial tumors exhibit reduced m6A methylation due to either mutation in METTL14 (~10%) or decreased expression of METTL3 (~60%). The reduction of mRNA m6A methylation through METTL14 mutation or METTL3 downregulation increased the proliferation and tumorigenicity of endometrial cancer cells in vitro and in vivo. RNA-seq and m6A-seq of human tumor tissue identified several transcripts in the Akt pathway exhibiting altered m6A methylation, including PHLPP2 and components of mTORC2. The altered stability and translation of these transcripts mediated the increased proliferation of cells with reduced mRNA m6A methylation. These results establish aberrant m6A methylation as a critical oncogenic mechanism underlying a majority of endometrial cancer.
 
Overall design m6A in endometrial cancer patient samples and HEC-1-A stable cell lines
 
Contributor(s) Liu J, Harada BT, Lu Z, He C
Citation(s) 30154548
Submission date Jan 22, 2017
Last update date May 15, 2019
Contact name zhike lu
E-mail(s) zhikelu@gmail.com
Organization name University of Chicago
Department department of chemistry
Street address 5801 South Ellis Avenue
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (32)
GSM2464900 1642 tumor adjacent-input
GSM2464901 1642 tumor adjacent-ip
GSM2464902 1642 tumor-input
Relations
BioProject PRJNA362818
SRA SRP097578

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Supplementary file Size Download File type/resource
GSE93911_RAW.tar 3.2 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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