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Series GSE96852 Query DataSets for GSE96852
Status Public on Mar 15, 2018
Title The roles of CSE1L in epigenetic silencing
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Epigenetic silencing can be mediated by various mechanisms and many regulators remain to be identified. We identified CSE1L as a factor essential for silencing of the reporter gene and a fraction of endogenous methylated genes. CSE1L depletion did not cause DNA demethylation or increase of global histone acetylation. Nevertheless, these methylated genes derepressed by CSE1L depletion largely overlapped with methylated genes that were also reactivated by treatment of histone deacetylase inhibitors (HDACi).
 
Overall design We measured DNA methylation by MeDIP and expression profiles by RNA-seq in B2-1 HEK293 cells and serveral Knockdown/treatment cells.
 
Contributor(s) Dong Q, Li X, Wang C, Xu S, Yuan G, Shao W, Huang H, Zheng Y, Wang H, Lei X, Zhang Z, Zhu B
Citation(s) 29636421
Submission date Mar 21, 2017
Last update date Mar 21, 2019
Contact name Zhuqiang Zhang
E-mail(s) zhangzhuqiang@ibp.ac.cn
Organization name Institute of Biophysics, CAS
Street address 15 Datun Road, Chaoyang District.
City Beijing
State/province ---
ZIP/Postal code 100101
Country China
 
Platforms (3)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
GPL20795 HiSeq X Ten (Homo sapiens)
GPL23227 BGISEQ-500 (Homo sapiens)
Samples (30)
GSM2545103 B2-1-RNA.rep1
GSM2545104 B2-1-RNA.rep2
GSM2545105 B2-1-5Aza-RNA.rep2
Relations
BioProject PRJNA379938
SRA SRP102231

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE96852_RAW.tar 2.5 Gb (http)(custom) TAR (of BIGWIG, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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