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Status |
Public on Mar 15, 2018 |
Title |
The roles of CSE1L in epigenetic silencing |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing Methylation profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing
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Summary |
Epigenetic silencing can be mediated by various mechanisms and many regulators remain to be identified. We identified CSE1L as a factor essential for silencing of the reporter gene and a fraction of endogenous methylated genes. CSE1L depletion did not cause DNA demethylation or increase of global histone acetylation. Nevertheless, these methylated genes derepressed by CSE1L depletion largely overlapped with methylated genes that were also reactivated by treatment of histone deacetylase inhibitors (HDACi).
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Overall design |
We measured DNA methylation by MeDIP and expression profiles by RNA-seq in B2-1 HEK293 cells and serveral Knockdown/treatment cells.
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Contributor(s) |
Dong Q, Li X, Wang C, Xu S, Yuan G, Shao W, Huang H, Zheng Y, Wang H, Lei X, Zhang Z, Zhu B |
Citation(s) |
29636421 |
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Submission date |
Mar 21, 2017 |
Last update date |
Mar 21, 2019 |
Contact name |
Zhuqiang Zhang |
E-mail(s) |
zhangzhuqiang@ibp.ac.cn
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Organization name |
Institute of Biophysics, CAS
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Street address |
15 Datun Road, Chaoyang District.
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City |
Beijing |
State/province |
--- |
ZIP/Postal code |
100101 |
Country |
China |
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Platforms (3) |
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Samples (30)
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Relations |
BioProject |
PRJNA379938 |
SRA |
SRP102231 |