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Series GSE97880 Query DataSets for GSE97880
Status Public on Jul 21, 2017
Title Medial Ganglionic Eminence and Cortical Organoids Model Human Brain Development and Interneuron Migration [ATAC-seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Organoid techniques provide unique platforms to model brain development and neurological disorders. While organoids recapitulating corticogenesis were established, a system modeling human medial ganglionic eminence (MGE) development, a critical ventral brain domain producing cortical interneurons and related lineages, remains to be developed. Here, we describe a system to generate MGE or cortex-specific organoids from human pluripotent stem cells. These organoids recapitulate the developments of MGE and cortex domains respectively. Population and single-cell transcriptomic profiling revealed transcriptional dynamics and lineage productions during MGE and cortical organoids development. Chromatin accessibility landscapes were found to be involved in this process. Furthermore, MGE and cortical organoids generated physiologically functional neurons and neuronal networks. Finally, we applied fusion organoids as a model to investigate human interneuron migration. Together, our study provides a new platform for generating domain-specific brain organoids, for modeling human interneuron migration, and offers deeper insight into molecular dynamics during human brain development.
 
Overall design Chromatin organization profiles of hMGEOs and hCOs were generated by deep sequencing
 
Contributor(s) Xiang Y, Tanaka Y, Park I
Citation(s) 28757360
Submission date Apr 17, 2017
Last update date May 15, 2019
Contact name Yangfei Xiang
E-mail(s) yangfei.xiang@yale.edu
Phone 2037375173
Organization name Yale University
Department Genetics
Lab Yale Stem Cell Center
Street address 10 Amistad St. Room 214J
City New Haven
State/province CT
ZIP/Postal code 06511
Country USA
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (3)
GSM2579550 hESC
GSM2579551 hMGEOs day72
GSM2579552 hCOs day72
This SubSeries is part of SuperSeries:
GSE97882 Medial Ganglionic Eminence and Cortical Organoids Model Human Brain Development and Interneuron Migration
Relations
BioProject PRJNA383297
SRA SRP104136

Download family Format
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Supplementary file Size Download File type/resource
GSE97880_RAW.tar 3.4 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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