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Series GSE98177 Query DataSets for GSE98177
Status Public on Apr 26, 2017
Title Transcriptome-wide analysis of IL2RA enhancer activation by CRISPRa
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We previously performed a tiling CRISPR activation (CRISPRa) screen in Jurkat T cells to discover enhancers controlling IL2RA expression. This screen identified six regions where recruitment of dCas9-VP64 was sufficient to drive increased expression of IL2RA. We named these putative enhancers CRISPRa Responsive Elements (CaREs). To examine transcriptome-wide consequences of dCas9-VP64 recruitment to IL2RA CaREs, we performed RNA-Seq on HuT78 cells stably expressing dCas9-VP64 and gRNAs targeting the IL2RA TSS, CaRE3, or CaRE4, or stimulated with anti-CD3/CD28 antibodies.
 
Overall design RNA-Seq of HuT78 cells stably expression dCas9-VP64 and sgRNAs targeting the IL2RA TSS, CaRE3, or CaRE4, or non-targeting control sgRNA. Stimulated and unstimulated HuT78 cells stably expressing dCas9-VP64 but no sgRNA were also analyzed for comparison.
 
Contributor(s) Corn J, Marson A
Citation(s) 28854172
Submission date Apr 25, 2017
Last update date Jul 25, 2021
Contact name Jacob Ellery Corn
E-mail(s) jacob.corn@gmail.com
Phone 510-664-7258
Organization name University of California, Berkeley
Department Innovative Genomics Institute
Lab Corn
Street address 2151 Berkeley Way
City Berkeley
State/province CA
ZIP/Postal code 94704
Country USA
 
Platforms (1)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
Samples (18)
GSM2588921 Non-targeting sgRNA 2 rep1
GSM2588922 Non-targeting sgRNA 2 rep2
GSM2588923 IL2RA TSS sgRNA 1 rep1
Relations
BioProject PRJNA384202
SRA SRP105197

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Supplementary file Size Download File type/resource
GSE98177_RAW.tar 38.2 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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