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Status |
Public on Apr 26, 2017 |
Title |
Transcriptome-wide analysis of IL2RA enhancer activation by CRISPRa |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We previously performed a tiling CRISPR activation (CRISPRa) screen in Jurkat T cells to discover enhancers controlling IL2RA expression. This screen identified six regions where recruitment of dCas9-VP64 was sufficient to drive increased expression of IL2RA. We named these putative enhancers CRISPRa Responsive Elements (CaREs). To examine transcriptome-wide consequences of dCas9-VP64 recruitment to IL2RA CaREs, we performed RNA-Seq on HuT78 cells stably expressing dCas9-VP64 and gRNAs targeting the IL2RA TSS, CaRE3, or CaRE4, or stimulated with anti-CD3/CD28 antibodies.
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Overall design |
RNA-Seq of HuT78 cells stably expression dCas9-VP64 and sgRNAs targeting the IL2RA TSS, CaRE3, or CaRE4, or non-targeting control sgRNA. Stimulated and unstimulated HuT78 cells stably expressing dCas9-VP64 but no sgRNA were also analyzed for comparison.
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Contributor(s) |
Corn J, Marson A |
Citation(s) |
28854172 |
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Submission date |
Apr 25, 2017 |
Last update date |
Jul 25, 2021 |
Contact name |
Jacob Ellery Corn |
E-mail(s) |
jacob.corn@gmail.com
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Phone |
510-664-7258
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Organization name |
University of California, Berkeley
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Department |
Innovative Genomics Institute
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Lab |
Corn
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Street address |
2151 Berkeley Way
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City |
Berkeley |
State/province |
CA |
ZIP/Postal code |
94704 |
Country |
USA |
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Platforms (1) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (18)
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Relations |
BioProject |
PRJNA384202 |
SRA |
SRP105197 |