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Status |
Public on May 11, 2017 |
Title |
A practical solution for preserving single cells for RNA sequencing |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
We present a method for histological tissue fixation using dithio-bis(succinimidyl propionate) (DSP), or Lomant's Reagent, to stabilise cell samples for single-cell transcriptomic applications.
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Overall design |
Single-cell RNAseq from 2,3 and 7 day old DSP fixed cells were compared with RNAseq from fresh cells
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Contributor(s) |
Sharma E, Attar M, Bowden R |
Citation(s) |
29391536 |
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Submission date |
May 09, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Eshita Sharma |
Organization name |
Wellcome Trust Centre for Human Genetics
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Department |
Bioinformatics
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Street address |
Roosevelt Drive, Headington
|
City |
Oxford |
State/province |
Oxfordshire |
ZIP/Postal code |
OX3 7BN |
Country |
United Kingdom |
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Platforms (2) |
GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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Samples (267)
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Relations |
BioProject |
PRJNA386155 |
SRA |
SRP106788 |