|
| Status |
Public on Jan 04, 2017 |
| Title |
ChIP-seq from HEK293 (ENCLB700PYI) |
| Sample type |
SRA |
| |
|
| Source name |
Homo sapiens HEK293 immortalized cell line derived from HEK293 stably expressing N-terminal eGFP-ZNF414 fusion protein
|
| Organism |
Homo sapiens |
| Characteristics |
antibody: eGFP-ZNF414
line: HEK293
biomaterial_type: immortalized cell line
description: HEK293 cell line stably expressing N-terminal tagged eGFP-ZNF414 under the control of a CMV promoter.
biosample encode accession: ENCBS852XCW (SAMN06122427)
age: unknown
dev stage: embryonic
Sex: female
health state: unknown
lab: Michael Snyder, Stanford
link: ENCBS852XCW at ENCODE; https://www.encodeproject.org/ENCBS852XCW/
link: Derived from ENCODE donor ENCDO000ABF; https://www.encodeproject.org/ENCDO000ABF/
link: Transfection protocol; https://www.encodeproject.org/documents/90e0e15b-ca37-4653-8c14-9748db04ae3f/@@download/attachment/Thermo_Fisher_Flp-In_system.png
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
general protocol: https://www.encodeproject.org/documents/0994a79b-44ae-49ef-a44c-6b2ebf85a230/@@download/attachment/ChIP-Seq%20Biorupter%20Pico%20BravoTruSeq%20protocol%20for%20Syapse%20%28modified%20for%20HEK293%20GFP-ZNF%29.pdf
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
https://www.encodeproject.org/experiments/ENCSR517GAU/
***************
biological replicate number: 1
technical replicate number: 1
description: eGFP-ZNF414 ChIP-seq on human HEK293 cells.
experiment encode accession: ENCSR517GAU
assay title: ChIP-seq
assembly: hg19, GRCh38
possible controls: ENCSR157LMX, ENCSR274OGE, ENCSR342AGU, ENCSR354FNX, ENCSR429LAP, ENCSR688NWK
encode release date: 2016-10-31
lab: Michael Snyder, Stanford
library encode accession: ENCLB700PYI
size range: 450-650
|
| Data processing |
See GSM*_README.txt supplementary file linked below
|
| |
|
| Submission date |
Dec 11, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
ENCODE DCC |
| E-mail(s) |
encode-help@lists.stanford.edu
|
| Organization name |
ENCODE DCC
|
| Street address |
300 Pasteur Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5120 |
| Country |
USA |
| |
|
| Platform ID |
GPL20301 |
| Series (1) |
| GSE91833 |
ChIP-seq from HEK293 (ENCSR517GAU) |
|
| Relations |
| BioSample |
SAMN06122427 |
| SRA |
SRX2424066 |
| Named Annotation |
GSM2423599_ENCFF074EKG_peaks_GRCh38.bed.gz |
| Named Annotation |
GSM2423599_ENCFF269HRB_signal_p-value_GRCh38.bigWig |
| Named Annotation |
GSM2423599_ENCFF315YPN_fold_change_over_control_GRCh38.bigWig |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM2423599_ENCFF074EKG_peaks_GRCh38.bed.gz |
6.8 Mb |
(ftp)(http) |
BED |
| GSM2423599_ENCFF144RKC_peaks_GRCh38.bigBed |
12.6 Mb |
(ftp)(http) |
BIGBED |
| GSM2423599_ENCFF203SNG_fold_change_over_control_hg19.bigWig |
1.6 Gb |
(ftp)(http) |
BIGWIG |
| GSM2423599_ENCFF269HRB_signal_p-value_GRCh38.bigWig |
1.3 Gb |
(ftp)(http) |
BIGWIG |
| GSM2423599_ENCFF315YPN_fold_change_over_control_GRCh38.bigWig |
1.6 Gb |
(ftp)(http) |
BIGWIG |
| GSM2423599_ENCFF324BXG_peaks_hg19.bed.gz |
6.8 Mb |
(ftp)(http) |
BED |
| GSM2423599_ENCFF354GRD_signal_p-value_hg19.bigWig |
1.5 Gb |
(ftp)(http) |
BIGWIG |
| GSM2423599_ENCFF574HBG_peaks_hg19.bigBed |
12.6 Mb |
(ftp)(http) |
BIGBED |
| GSM2423599_ENCLB700PYI_README.txt.gz |
1.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
| Processed data provided as supplementary file |
