|
| Status |
Public on Mar 30, 2022 |
| Title |
Sarcoidosis patient 8 after treated with tofacitinib for six months [Olink Explore 1536 panel] |
| Sample type |
protein |
| |
|
| Source name |
Blood
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: Sarcoidosis patient
treatment: tofacitinib
tissue: Blood
|
| Treatment protocol |
9 patients with severe, long-standing sarcoidosis were enrolled and treated with tofacitinib monotherapy (5 mg twice daily) for 6 months. Concomitant methotrexate and/or low dose prednisone was allowed so long as the dose had been stable over the prior 3 months.
|
| Extracted molecule |
protein |
| Extraction protocol |
Blood samples were collected from participants at baseline and again after 6 months of therapy. Samples were compared to 11 healthy, roughly age matched controls. Blood was collected in EDTA-coated tubes and centrifuged; the buffy coat was removed and stored separately from the plasma, which was aliquoted and stored at -80ºC.
|
| Label |
NOT PROVIDED
|
| Label protocol |
according to manufacture protocol
|
| |
|
| Hybridization protocol |
according to manufacture protocol
|
| Scan protocol |
according to manufacture protocol
|
| Data processing |
Plasma was analyzed using the Olink Explore 1536 panel. NPX, Normalized Protein eXpression, is Olink's arbitrary unit which is in Log2 scale. It is calculated from Ct values and data pre-processing (normalization) is performed to minimize both intra- and inter-assay variation. NPX data allows users to identify changes for individual protein levels across their sample set, and then use this data to establish protein signatures:
https://www.olink.com/question/what-is-npx/#:~:text=The%20NPX%20scale%20is%20inverted,%3A%202NPX%3D%20linear%20NPX
|
| |
|
| Submission date |
Mar 18, 2021 |
| Last update date |
Mar 30, 2022 |
| Contact name |
William Damsky |
| E-mail(s) |
william.damsky@yale.edu
|
| Organization name |
Yale School of Medicine
|
| Street address |
333 Cedar St
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
|
| Platform ID |
GPL29882 |
| Series (2) |
| GSE169148 |
Evaluation of tofacitinib in cutaneous sarcoidosis [Olink Explore 1536] |
| GSE169149 |
Evaluation of tofacitinib in cutaneous sarcoidosis |
|
