MCF7 cells were grown in conditions devoid of estrogen for 48h - (IMEM media supplemented w/ 10% Charcoal Stripped Serum) at time 0h cells were treated with 10-8 M estradiol (E2) - This SAGE library represents the 10h time-point of E2 treatment Sex: Female Organ: Breast Cell_type: epithelial Cell_line: MCF7 Lab host: Top10 bacteria Vector: pZero Vector type: plasmid R. Site 1: NlaIII R. Site 2: BsmF1 as tagging enzyme Sequences generated to date: 2324
This library represents a Cancer Genome Anatomy Project library, which was either produced through CGAP funding, or donated to CGAP. The Cancer Genome Anatomy Project (CGAP: http://cgap.nci.nih.gov) is an interdisciplinary program established and administered by the National Cancer Institute (NCI: http://www.nci.nih.gov) to generate the information and technological tools needed to decipher the molecular anatomy of the cancer cell. Keywords = mammary gland Keywords = carcinoma Keywords = cell line Keywords = estrogen Keywords = EST
