Introduction
IgBLAST was developed at NCBI to facilitate analysis of immunoglobulin as well as T cell receptor (TR) sequences. It uses BLAST search algorithm.
In addition to performing a regular BLAST search, IgBLAST has several additional functions:
- Reports the germline V, D, J and C gene matches to the query sequence.
- Annotates the immunoglobulin domains (FR1 through FR4 as well as C region).
- Reveals the V(D)J junction details such as nucleotide homology between the ends of V(D)J segments and N nucleotide insertions.
- Indicates the whether the rearrangement is in-frame or out-of-frame.
D and J gene reporting is only for nucleotide sequence search.
Example 1
The following is an example searching a human IgH nucleotide sequence (GenBank record MW176150) against the Ig germline V, D, J and C gene databases.
The first part of the report is the summary match information between query sequence and the top matched germline V, D, J and C genes. Note that the in-frame rearrangement is defined as the last coding triplet of V gene coding frame being in frame with the first coding triplet of J gene coding frame.
The second part is the detailed alignments between query and germline hits.
The far left column indicates the gene category (V, D, J or C) for the hits. The second column shows the percent identity between query and the hit (The number of matches and the alignment length are indicated in parenthesis. Any gap is counted as a mismatch and is included in alignment length).
The start and end positions of the nucleotides or amino acids are indicated for each line of alignment. A dot "." indicates sequence identity to the query. A dash "-" indicates a gap in the sequence.
Domain classification requested: imgt
V-(D)-J rearrangement summary for query sequence (multiple equivalent top matches, if present, are separated by a comma):
| Top V gene match | Top D gene match | Top J gene match | Top C gene match | Chain type | stop codon | V-J frame | Productive | Strand | V frame shift |
| IGHV1-18*01 | IGHD4-23*01 | IGHJ4*02 | IGHG1 | VH | No | In-frame | Yes | + | No |
V-(D)-J junction details based on top germline gene matches:
| V region end | V-D junction* | D region | D-J junction* | J region start |
| GAGAG | TCA | ACGGTGGTAAC | CCGGGATGG | TTTGA |
*: Overlapping nucleotides may exist at V-D-J junction (i.e, nucleotides that could be assigned
to either rearranging gene). Such nucleotides are indicated inside a parenthesis (i.e., (TACAT))
but are not included under the V, D or J gene itself.
Sub-region sequence details:
| | Nucleotide sequence | Translation | Start | End |
| CDR3 | GCGAGAGTCAACGGTGGTAACCCGGGATGGTTTGACTAC | ARVNGGNPGWFDY | 288 | 326 |
Alignment summary between query and top germline V gene hit:
| | from | to | length | matches | mismatches | gaps | identity(%) |
| FR1-IMGT | 1 | 74 | 74 | 72 | 2 | 0 | 97.3 |
| CDR1-IMGT | 75 | 98 | 24 | 18 | 6 | 0 | 75 |
| FR2-IMGT | 99 | 149 | 51 | 48 | 3 | 0 | 94.1 |
| CDR2-IMGT | 150 | 173 | 24 | 24 | 0 | 0 | 100 |
| FR3-IMGT | 174 | 287 | 114 | 106 | 8 | 0 | 93 |
| CDR3-IMGT (germline) | 288 | 294 | 7 | 7 | 0 | 0 | 100 |
| Total | | | 294 | 275 | 19 | 0 | 93.5 |
Alignments
<--------------------------------FR1-IMGT--------------------------------><-------CDR1-IMG
V Q L V Q S G A E V K K P G A S V K V P C K A S G Y S Y I
MW176150.1 1 AGGTTCAACTGGTGCAGTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCCCCTGCAAGGCTTCTGGTTACAGTTATATCA 90
V 93.5% (275/294) IGHV1-18*01 2 .......G...................................................T.....................CC.T..C.. 91
V Q L V Q S G A E V K K P G A S V K V S C K A S G Y T F T
V 93.2% (274/294) IGHV1-18*03 2 .......G...................................................T.....................CC.T..C.. 91
V 93.2% (274/294) IGHV1-18*04 2 .......G...................................................T.....................CC.T..C.. 91
T------><--------------------FR2-IMGT---------------------><-------CDR2-IMGT------><------
S H G L T W V R Q A P G Q G L E W M G W I S A Y N G N T K S
MW176150.1 91 GTCATGGTCTCACCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAGCGCTTACAATGGTAACACAAAGTCTG 180
V 93.5% (275/294) IGHV1-18*01 92 .CT.....A...G..........................................A.............................C.A.. 181
S Y G I S W V R Q A P G Q G L E W M G W I S A Y N G N T N Y
V 93.2% (274/294) IGHV1-18*03 92 .CT.....A...G..........................................A.............................C.A.. 181
V 93.2% (274/294) IGHV1-18*04 92 .CT.C...A...G..........................................A.............................C.A.. 181
----------------------------------------------FR3-IMGT------------------------------------
A Q K F K G R V T M T T D T S T S T A Y M E L R S L R S D D
MW176150.1 181 CACAGAAGTTCAAGGGCAGAGTCACCATGACGACAGACACGTCCACGAGTACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACA 270
V 93.5% (275/294) IGHV1-18*01 182 ........C..C...................C........A........C........................................ 271
A Q K L Q G R V T M T T D T S T S T A Y M E L R S L R S D D
V 93.2% (274/294) IGHV1-18*03 182 ........C..C...................C........A........C........................................ 271
V 93.2% (274/294) IGHV1-18*04 182 ........C..C...................C........A........C........................................ 271
----------------><--------------CDR3-IMGT--------------><-----------FR4-IMGT------------><
T A V Y Y C A R V N G G N P G W F D Y W G Q G T L V T V S S
MW176150.1 271 CGGCCGTCTATTACTGTGCGAGAGTCAACGGTGGTAACCCGGGATGGTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 360
V 93.5% (275/294) IGHV1-18*01 272 .......G................------------------------------------------------------------------ 295
T A V Y Y C A R
V 93.2% (274/294) IGHV1-18*03 272 T......G................------------------------------------------------------------------ 295
V 93.2% (274/294) IGHV1-18*04 272 .......G................------------------------------------------------------------------ 295
D 100.0% (11/11) IGHD4-23*01 6 ---------------------------...........---------------------------------------------------- 16
D 100.0% (7/7) IGHD2-15*01 14 -----------------------------.......------------------------------------------------------ 20
D 100.0% (6/6) IGHD2-21*01 11 -----------------------------......------------------------------------------------------- 16
J 100.0% (43/43) IGHJ4*02 6 -----------------------------------------------........................................... 48
J 97.7% (42/43) IGHJ4*01 6 -----------------------------------------------.................A......................... 48
J 93.5% (43/46) IGHJ5*01 6 --------------------------------------------.....C....C.........A......................... 51
C 99.3% (978/985) IGHG1 1 -----------------------------------------------------------------------------------------. 1
C 94.7% (933/985) IGHG2 1 -----------------------------------------------------------------------------------------. 1
------------------------------------------------------------------------------------------
A S T K G P S V F P L A P S S K S T S G G T A A L G C L V K
MW176150.1 361 CCTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGG 450
C 99.3% (978/985) IGHG1 2 ...................................................................A...................... 91
C 94.7% (933/985) IGHG2 2 ..................................G....G.....G.........C.A.A.............................. 91
------------------------------------------------------------------------------------------
D Y F P E P V T V S W N S G A L T S G V H T F P A V L Q S S
MW176150.1 451 ACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAG 540
C 99.3% (978/985) IGHG1 92 .......................................................................................... 181
C 94.7% (933/985) IGHG2 92 ...........................................T.............................................. 181
------------------------------------------------------------------------------------------
G L Y S L S S V V T V P S S S L G T Q T Y I C N V N H K P S
MW176150.1 541 GACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCA 630
C 99.3% (978/985) IGHG1 182 .......................................................................................... 271
C 94.7% (933/985) IGHG2 182 ..........................................A...C................C.........AG............... 271
------------------------------------------------------------------------------------------
N T K V D K R V E P K S C D K T H T C P P C P A P E L L G G
MW176150.1 631 ACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGAC 720
C 99.3% (978/985) IGHG1 272 ..................A....................................................................... 361
C 94.7% (933/985) IGHG2 272 ..................C........G.....G.....T.G.G---------....................---..CTG...CA.... 349
------------------------------------------------------------------------------------------
P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S
MW176150.1 721 CGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCC 810
C 99.3% (978/985) IGHG1 362 .......................................................................................... 451
C 94.7% (933/985) IGHG2 350 ...................................................................G...................... 439
-------------------------------------C region---------------------------------------------
H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N
MW176150.1 811 ACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACA 900
C 99.3% (978/985) IGHG1 452 .......................................................................................... 541
C 94.7% (933/985) IGHG2 440 ..........C......C....................................................A.............T..... 529
------------------------------------------------------------------------------------------
S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A
MW176150.1 901 GCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCC 990
C 99.3% (978/985) IGHG1 542 .......................................................................................... 631
C 94.7% (933/985) IGHG2 530 ......T.........................G...................C..................................G.. 619
------------------------------------------------------------------------------------------
L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R E E
MW176150.1 991 TCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGA 1080
C 99.3% (978/985) IGHG1 632 .....................................................................................T...C 721
C 94.7% (933/985) IGHG2 620 ................................A......................................................... 709
------------------------------------------------------------------------------------------
M T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P
MW176150.1 1081 TGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGG 1170
C 99.3% (978/985) IGHG1 722 .......................................................................................... 811
C 94.7% (933/985) IGHG2 710 ..............................................C............T.............................. 799
------------------------------------------------------------------------------------------
E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W
MW176150.1 1171 AGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGC 1260
C 99.3% (978/985) IGHG1 812 ..........................................................C............................... 901
C 94.7% (933/985) IGHG2 800 ...................A......A...............................C............................... 889
----------------------------------------------------------------------------------->
Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P
MW176150.1 1261 AGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCCCCGG 1344
C 99.3% (978/985) IGHG1 902 ..........................................................A....................T.... 985
C 94.7% (933/985) IGHG2 890 ..........................................................A....................T.... 973
Example 2
This example is the same as above except that an additional database (nr) is selected.
Alignments
<--------------------------------FR1-IMGT--------------------------------><-------CDR1-IMG
V Q L V Q S G A E V K K P G A S V K V P C K A S G Y S Y I
MW176150.1 1 AGGTTCAACTGGTGCAGTCTGGAGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCCCCTGCAAGGCTTCTGGTTACAGTTATATCA 90
V 93.5% (275/294) IGHV1-18*01 2 .......G...................................................T.....................CC.T..C.. 91
V Q L V Q S G A E V K K P G A S V K V S C K A S G Y T F T
V 93.2% (274/294) IGHV1-18*03 2 .......G...................................................T.....................CC.T..C.. 91
V 93.2% (274/294) IGHV1-18*04 2 .......G...................................................T.....................CC.T..C.. 91
100.0% (294/294) MW176150.1 1 .......................................................................................... 90
94.6% (278/294) MW654763.1 2 .......G...................................................T.....................CC.T..... 91
94.2% (277/294) MW176535.1 1 .......G...................................................T.....................T..T..C.. 90
94.2% (277/294) KF994189.1 2 .......G...................................................T.........T...........CC.T..C.. 91
93.9% (276/294) KX387061.1 2 ...........................................................T.....................CC.T..C.. 91
93.9% (276/294) MN284221.1 2 ...........................................................T.....................CC.T..C.. 91
93.9% (276/294) MN287921.1 2 .......G...................................................T......................C.T..C.. 91
93.9% (276/294) JX432840.1 2 ................C..........................................T........A............C..T..... 91
93.9% (275/293) MN280855.1 2 .......G...................................................T.....................CC.T..C.. 91
93.5% (275/294) OM457736.1 2 .......G...................................................T.....................CC.T..C.. 91
T------><--------------------FR2-IMGT---------------------><-------CDR2-IMGT------><------
S H G L T W V R Q A P G Q G L E W M G W I S A Y N G N T K S
MW176150.1 91 GTCATGGTCTCACCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGGTGGATCAGCGCTTACAATGGTAACACAAAGTCTG 180
V 93.5% (275/294) IGHV1-18*01 92 .CT.....A...G..........................................A.............................C.A.. 181
S Y G I S W V R Q A P G Q G L E W M G W I S A Y N G N T N Y
V 93.2% (274/294) IGHV1-18*03 92 .CT.....A...G..........................................A.............................C.A.. 181
V 93.2% (274/294) IGHV1-18*04 92 .CT.C...A...G..........................................A.............................C.A.. 181
100.0% (294/294) MW176150.1 91 .......................................................................................... 180
94.6% (278/294) MW654763.1 92 ..T.....A...A........................................................................C.A.. 181
94.2% (277/294) MW176535.1 91 ..T.....A..............................................A................G............C.A.. 180
94.2% (277/294) KF994189.1 92 .CT.....A...G..........................................A.............................C.A.. 181
93.9% (276/294) KX387061.1 92 ..T.....A...G.........................................CA................C............T.A.. 181
93.9% (276/294) MN284221.1 92 .CT.....A...G..........................................A.............................C.A.. 181
93.9% (276/294) MN287921.1 92 .CT.....A....T.........................................A.............................C.A.. 181
93.9% (276/294) JX432840.1 92 .CT....AG...G...........................C......................................T.....C.A.. 181
93.9% (275/293) MN280855.1 92 .CT.....A..............................................A.............................C.A.. 181
93.5% (275/294) OM457736.1 92 .CT.....A...G..........................................A.............................C.A.. 181
----------------------------------------------FR3-IMGT------------------------------------
A Q K F K G R V T M T T D T S T S T A Y M E L R S L R S D D
MW176150.1 181 CACAGAAGTTCAAGGGCAGAGTCACCATGACGACAGACACGTCCACGAGTACAGCCTACATGGAGCTGAGGAGCCTGAGATCTGACGACA 270
V 93.5% (275/294) IGHV1-18*01 182 ........C..C...................C........A........C........................................ 271
A Q K L Q G R V T M T T D T S T S T A Y M E L R S L R S D D
V 93.2% (274/294) IGHV1-18*03 182 ........C..C...................C........A........C........................................ 271
V 93.2% (274/294) IGHV1-18*04 182 ........C..C...................C........A........C........................................ 271
100.0% (294/294) MW176150.1 181 .......................................................................................... 270
94.6% (278/294) MW654763.1 182 ...........C...................C........A........C.............................G.......... 271
94.2% (277/294) MW176535.1 181 ........C..C...................C........A........C........................................ 270
94.2% (277/294) KF994189.1 182 ...........C...................C.................C........................................ 271
93.9% (276/294) KX387061.1 182 ...........C.................T.C........A........C........................................ 271
93.9% (276/294) MN284221.1 182 ........C..C............................A........C.......................................T 271
93.9% (276/294) MN287921.1 182 ...........C...................C........A.......AC........................................ 271
93.9% (276/294) JX432840.1 182 ...........C...................C........A................................................. 271
93.9% (275/293) MN280855.1 182 ........C..C...................C........A........C........................................ 271
93.5% (275/294) OM457736.1 182 ........C..C...................C........A........C........................................ 271
----------------><--------------CDR3-IMGT--------------><-----------FR4-IMGT------------><
T A V Y Y C A R V N G G N P G W F D Y W G Q G T L V T V S S
MW176150.1 271 CGGCCGTCTATTACTGTGCGAGAGTCAACGGTGGTAACCCGGGATGGTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG 360
V 93.5% (275/294) IGHV1-18*01 272 .......G................------------------------------------------------------------------ 295
T A V Y Y C A R
V 93.2% (274/294) IGHV1-18*03 272 T......G................------------------------------------------------------------------ 295
V 93.2% (274/294) IGHV1-18*04 272 .......G................------------------------------------------------------------------ 295
D 100.0% (11/11) IGHD4-23*01 6 ---------------------------...........---------------------------------------------------- 16
D 100.0% (7/7) IGHD2-15*01 14 -----------------------------.......------------------------------------------------------ 20
D 100.0% (6/6) IGHD2-21*01 11 -----------------------------......------------------------------------------------------- 16
J 100.0% (43/43) IGHJ4*02 6 -----------------------------------------------........................................... 48
J 97.7% (42/43) IGHJ4*01 6 -----------------------------------------------.................A......................... 48
J 93.5% (43/46) IGHJ5*01 6 --------------------------------------------.....C....C.........A......................... 51
C 99.3% (978/985) IGHG1 1 -----------------------------------------------------------------------------------------. 1
C 94.7% (933/985) IGHG2 1 -----------------------------------------------------------------------------------------. 1
100.0% (294/294) MW176150.1 271 ........................------------------------------------------------------------------ 294
94.6% (278/294) MW654763.1 272 .......G................------------------------------------------------------------------ 295
94.2% (277/294) MW176535.1 271 .......G................------------------------------------------------------------------ 294
94.2% (277/294) KF994189.1 272 ........................------------------------------------------------------------------ 295
93.9% (276/294) KX387061.1 272 ........................------------------------------------------------------------------ 295
93.9% (276/294) MN284221.1 272 .......A................------------------------------------------------------------------ 295
93.9% (276/294) MN287921.1 272 .......G................------------------------------------------------------------------ 295
93.9% (276/294) JX432840.1 272 .......G................------------------------------------------------------------------ 295
93.9% (275/293) MN280855.1 272 .......G...............------------------------------------------------------------------- 294
93.5% (275/294) OM457736.1 272 .......G................------------------------------------------------------------------ 295
------------------------------------------------------------------------------------------
A S T K G P S V F P L A P S S K S T S G G T A A L G C L V K
MW176150.1 361 CCTCCACCAAGGGCCCATCGGTCTTCCCCCTGGCACCCTCCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGG 450
C 99.3% (978/985) IGHG1 2 ...................................................................A...................... 91
C 94.7% (933/985) IGHG2 2 ..................................G....G.....G.........C.A.A.............................. 91
------------------------------------------------------------------------------------------
D Y F P E P V T V S W N S G A L T S G V H T F P A V L Q S S
MW176150.1 451 ACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGCCCTGACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAG 540
C 99.3% (978/985) IGHG1 92 .......................................................................................... 181
C 94.7% (933/985) IGHG2 92 ...........................................T.............................................. 181
------------------------------------------------------------------------------------------
G L Y S L S S V V T V P S S S L G T Q T Y I C N V N H K P S
MW176150.1 541 GACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGCCCAGCA 630
C 99.3% (978/985) IGHG1 182 .......................................................................................... 271
C 94.7% (933/985) IGHG2 182 ..........................................A...C................C.........AG............... 271
------------------------------------------------------------------------------------------
N T K V D K R V E P K S C D K T H T C P P C P A P E L L G G
MW176150.1 631 ACACCAAGGTGGACAAGAGAGTTGAGCCCAAATCTTGTGACAAAACTCACACATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGAC 720
C 99.3% (978/985) IGHG1 272 ..................A....................................................................... 361
C 94.7% (933/985) IGHG2 272 ..................C........G.....G.....T.G.G---------....................---..CTG...CA.... 349
------------------------------------------------------------------------------------------
P S V F L F P P K P K D T L M I S R T P E V T C V V V D V S
MW176150.1 721 CGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTGAGCC 810
C 99.3% (978/985) IGHG1 362 .......................................................................................... 451
C 94.7% (933/985) IGHG2 350 ...................................................................G...................... 439
-------------------------------------C region---------------------------------------------
H E D P E V K F N W Y V D G V E V H N A K T K P R E E Q Y N
MW176150.1 811 ACGAAGACCCTGAGGTCAAGTTCAACTGGTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAACA 900
C 99.3% (978/985) IGHG1 452 .......................................................................................... 541
C 94.7% (933/985) IGHG2 440 ..........C......C....................................................A.............T..... 529
------------------------------------------------------------------------------------------
S T Y R V V S V L T V L H Q D W L N G K E Y K C K V S N K A
MW176150.1 901 GCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAAAGCCC 990
C 99.3% (978/985) IGHG1 542 .......................................................................................... 631
C 94.7% (933/985) IGHG2 530 ......T.........................G...................C..................................G.. 619
------------------------------------------------------------------------------------------
L P A P I E K T I S K A K G Q P R E P Q V Y T L P P S R E E
MW176150.1 991 TCCCAGCCCCCATCGAGAAAACCATCTCCAAAGCCAAAGGGCAGCCCCGAGAACCACAGGTGTACACCCTGCCCCCATCCCGGGAGGAGA 1080
C 99.3% (978/985) IGHG1 632 .....................................................................................T...C 721
C 94.7% (933/985) IGHG2 620 ................................A......................................................... 709
------------------------------------------------------------------------------------------
M T K N Q V S L T C L V K G F Y P S D I A V E W E S N G Q P
MW176150.1 1081 TGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGG 1170
C 99.3% (978/985) IGHG1 722 .......................................................................................... 811
C 94.7% (933/985) IGHG2 710 ..............................................C............T.............................. 799
------------------------------------------------------------------------------------------
E N N Y K T T P P V L D S D G S F F L Y S K L T V D K S R W
MW176150.1 1171 AGAACAACTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCTATAGCAAGCTCACCGTGGACAAGAGCAGGTGGC 1260
C 99.3% (978/985) IGHG1 812 ..........................................................C............................... 901
C 94.7% (933/985) IGHG2 800 ...................A......A...............................C............................... 889
----------------------------------------------------------------------------------->
Q Q G N V F S C S V M H E A L H N H Y T Q K S L S L S P
MW176150.1 1261 AGCAGGGGAACGTCTTCTCATGCTCCGTGATGCATGAGGCTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCCCCGG 1344
C 99.3% (978/985) IGHG1 902 ..........................................................A....................T.... 985
C 94.7% (933/985) IGHG2 890 ..........................................................A....................T.... 973
Last modified: Tue Feb 22 12:26:40 EST 2022